Nucleic Acids Research, 2000, Vol. 28, No. 18 3684-3693
© 2000 Oxford University Press
Two novel human and mouse DNA polymerases of the polX family
INSERM U373, Faculté de Médecine Necker-Enfants Malades, Université Paris V, 156 rue de Vaugirard, 75730 Paris cedex 15, France
We describe here two novel mouse and human DNA polymerases: one (pol 
) has homology with DNA polymerase ß while the other one (pol µ) is closer to terminal deoxynucleotidyltransferase. However both have DNA polymerase activity in vitro and share similar structural organization, including a BRCT domain, helix–loop–helix DNA-binding motifs and polymerase X domain. mRNA expression of pol is highest in testis and fetal liver, while expression of pol µ is more lymphoid, with highest expression both in thymus and tonsillar B cells. An unusually large number of splice variants is observed for the pol µ gene, most of which affect the polymerase domain. Expression of mRNA of both polymerases is down-regulated upon treatment by DNA damaging agents (UV light, 
-rays or H2O2). This suggests that their biological function may differ from DNA translesion synthesis, for which several DNA polymerase activities have been recently described. Possible functions are discussed.
* To whom correspondence should be addressed. Tel: +33 1 40 61 56 83; Fax: +33 1 40 61 55 90; Email: reynaud@infobiogen.fr Present addresses: Laurentiu Cocea, Amgen Research Institute, 620 University Avenue, Suite 706, Toronto, Ontario M5G 2C1, Canada Neetu Gupta, CNRS UMR 1773, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris cedex 15, France +AF161019, AF176097, AF176098 and AF176099
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  F. Romain, I. Barbosa, J. Gouge, F. Rougeon, and M. Delarue Conferring a template-dependent polymerase activity to terminal deoxynucleotidyltransferase by mutations in the Loop1 region Nucleic Acids Res., June 5, 2009; (2009) gkp460v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Maga, E. Crespan, U. Wimmer, B. van Loon, A. Amoroso, C. Mondello, C. Belgiovine, E. Ferrari, G. Locatelli, G. Villani, et al. Replication protein A and proliferating cell nuclear antigen coordinate DNA polymerase selection in 8-oxo-guanine repair PNAS, December 30, 2008; 105(52): 20689 - 20694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Davis, J. M. Havener, and D. A. Ramsden End-bridging is required for pol {micro} to efficiently promote repair of noncomplementary ends by nonhomologous end joining Nucleic Acids Res., May 1, 2008; 36(9): 3085 - 3094. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kubota, S. Maezawa, K. Koiwai, T. Hayano, and O. Koiwai Identification of functional domains in TdIF1 and its inhibitory mechanism for TdT activity Genes Cells, August 1, 2007; 12(8): 941 - 959. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-P. Capp, F. Boudsocq, A.-G. Besnard, B. S. Lopez, C. Cazaux, J.-S. Hoffmann, and Y. Canitrot Involvement of DNA polymerase {micro} in the repair of a specific subset of DNA double-strand breaks in mammalian cells Nucleic Acids Res., June 28, 2007; 35(11): 3551 - 3560. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. W. Duym, K. A. Fiala, N. Bhatt, and Z. Suo Kinetic Effect of a Downstream Strand and Its 5'-Terminal Moieties on Single Nucleotide Gap-filling Synthesis Catalyzed by Human DNA Polymerase {lambda} J. Biol. Chem., November 24, 2006; 281(47): 35649 - 35655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Fiala, W. W. Duym, J. Zhang, and Z. Suo Up-regulation of the Fidelity of Human DNA Polymerase {lambda} by Its Non-enzymatic Proline-rich Domain J. Biol. Chem., July 14, 2006; 281(28): 19038 - 19044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Takeuchi, T. Ishidoh, H. Iijima, I. Kuriyama, N. Shimazaki, O. Koiwai, K. Kuramochi, S. Kobayashi, F. Sugawara, K. Sakaguchi, et al. Structural relationship of curcumin derivatives binding to the BRCT domain of human DNA polymerase {lambda} Genes Cells, March 1, 2006; 11(3): 223 - 235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. K. Braithwaite, P. S. Kedar, L. Lan, Y. Y. Polosina, K. Asagoshi, V. P. Poltoratsky, J. K. Horton, H. Miller, G. W. Teebor, A. Yasui, et al. DNA Polymerase {lambda} Protects Mouse Fibroblasts against Oxidative DNA Damage and Is Recruited to Sites of DNA Damage/Repair J. Biol. Chem., September 9, 2005; 280(36): 31641 - 31647. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Shimazaki, T. Yazaki, T. Kubota, A. Sato, A. Nakamura, S. Kurei, S. Toji, K. Tamai, and O. Koiwai DNA polymerase lambda directly binds to proliferating cell nuclear antigen through its confined C-terminal region Genes Cells, July 1, 2005; 10(7): 705 - 715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Bebenek, M. Garcia-Diaz, S. R. Patishall, and T. A. Kunkel Biochemical Properties of Saccharomyces cerevisiae DNA Polymerase IV J. Biol. Chem., May 20, 2005; 280(20): 20051 - 20058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. K. Braithwaite, R. Prasad, D. D. Shock, E. W. Hou, W. A. Beard, and S. H. Wilson DNA Polymerase {lambda} Mediates a Back-up Base Excision Repair Activity in Extracts of Mouse Embryonic Fibroblasts J. Biol. Chem., May 6, 2005; 280(18): 18469 - 18475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Avkin, M. Goldsmith, S. Velasco-Miguel, N. Geacintov, E. C. Friedberg, and Z. Livneh Quantitative Analysis of Translesion DNA Synthesis across a Benzo[a]pyrene-Guanine Adduct in Mammalian Cells: THE ROLE OF DNA POLYMERASE {kappa} J. Biol. Chem., December 17, 2004; 279(51): 53298 - 53305. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Livingston, A. von Niederhausern, A. G. Jegga, D. C. Crawford, C. S. Carlson, M. J. Rieder, S. Gowrisankar, B. J. Aronow, R. B. Weiss, and D. A. Nickerson Pattern of Sequence Variation Across 213 Environmental Response Genes Genome Res., October 1, 2004; 14(10a): 1821 - 1831. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.-H. Thai and J. F. Kearney Distinct and Opposite Activities of Human Terminal Deoxynucleotidyltransferase Splice Variants J. Immunol., September 15, 2004; 173(6): 4009 - 4019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. E. Repasky, E. Corbett, C. Boboila, and D. G. Schatz Mutational Analysis of Terminal Deoxynucleotidyltransferase- Mediated N-Nucleotide Addition in V(D)J Recombination J. Immunol., May 1, 2004; 172(9): 5478 - 5488. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zariwala, W. K. O'Neal, P. G. Noone, M. W. Leigh, M. R. Knowles, and L. E. Ostrowski Investigation of the Possible Role of a Novel Gene, DPCD, in Primary Ciliary Dyskinesia Am. J. Respir. Cell Mol. Biol., April 1, 2004; 30(4): 428 - 434. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Covo, L. Blanco, and Z. Livneh Lesion Bypass by Human DNA Polymerase {micro} Reveals a Template-dependent, Sequence-independent Nucleotidyl Transferase Activity J. Biol. Chem., January 9, 2004; 279(2): 859 - 865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Lee, L. Blanco, T. Zhou, M. Garcia-Diaz, K. Bebenek, T. A. Kunkel, Z. Wang, and L. F. Povirk Implication of DNA Polymerase {lambda} in Alignment-based Gap Filling for Nonhomologous DNA End Joining in Human Nuclear Extracts J. Biol. Chem., January 2, 2004; 279(1): 805 - 811. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Cabelof, Z. Guo, J. J. Raffoul, R. W. Sobol, S. H. Wilson, A. Richardson, and A. R. Heydari Base Excision Repair Deficiency Caused by Polymerase {beta} Haploinsufficiency: Accelerated DNA Damage and Increased Mutational Response to Carcinogens Cancer Res., September 15, 2003; 63(18): 5799 - 5807. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Bebenek, M. Garcia-Diaz, L. Blanco, and T. A. Kunkel The Frameshift Infidelity of Human DNA Polymerase {lambda}: IMPLICATIONS FOR FUNCTION J. Biol. Chem., September 5, 2003; 278(36): 34685 - 34690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Marini, N. Kim, A. Schuffert, and R. D. Wood POLN, a Nuclear PolA Family DNA Polymerase Homologous to the DNA Cross-link Sensitivity Protein Mus308 J. Biol. Chem., August 22, 2003; 278(34): 32014 - 32019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Nick McElhinny and D. A. Ramsden Polymerase Mu Is a DNA-Directed DNA/RNA Polymerase Mol. Cell. Biol., April 1, 2003; 23(7): 2309 - 2315. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. V. Shcherbakova, K. Bebenek, and T. A. Kunkel Functions of Eukaryotic DNA Polymerases Sci. Aging Knowl. Environ., February 26, 2003; 2003(8): re3 - 3. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Maga, G. Villani, K. Ramadan, I. Shevelev, N. T. Le Gac, L. Blanco, G. Blanca, S. Spadari, and U. Hubscher Human DNA Polymerase lambda Functionally and Physically Interacts with Proliferating Cell Nuclear Antigen in Normal and Translesion DNA Synthesis J. Biol. Chem., December 6, 2002; 277(50): 48434 - 48440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-M. Tseng and A. E. Tomkinson A Physical and Functional Interaction between Yeast Pol4 and Dnl4-Lif1 Links DNA Synthesis and Ligation in Nonhomologous End Joining J. Biol. Chem., November 15, 2002; 277(47): 45630 - 45637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, X. Wu, D. Guo, O. Rechkoblit, J.-S. Taylor, N. E. Geacintov, and Z. Wang Lesion Bypass Activities of Human DNA Polymerase {micro} J. Biol. Chem., November 8, 2002; 277(46): 44582 - 44587. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Chiu, L. Pan, Z. Li, S. Ely, A. Chadburn, and D. M. Knowles DNA Polymerase {micro} Gene Expression in B-Cell Non-Hodgkin's Lymphomas : An Analysis Utilizing in Situ Hybridization Am. J. Pathol., October 1, 2002; 161(4): 1349 - 1355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. N. Mahajan, S. A. Nick McElhinny, B. S. Mitchell, and D. A. Ramsden Association of DNA Polymerase {micro} (pol {micro}) with Ku and Ligase IV: Role for pol {micro} in End-Joining Double-Strand Break Repair Mol. Cell. Biol., July 15, 2002; 22(14): 5194 - 5202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-B. Duvauchelle, L. Blanco, R. P. P. Fuchs, and A. M. Cordonnier Human DNA polymerase mu (Pol {micro}) exhibits an unusual replication slippage ability at AAF lesion Nucleic Acids Res., May 1, 2002; 30(9): 2061 - 2067. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kobayashi, M. Watanabe, Y. Okada, H. Sawa, H. Takai, M. Nakanishi, Y. Kawase, H. Suzuki, K. Nagashima, K. Ikeda, et al. Hydrocephalus, Situs Inversus, Chronic Sinusitis, and Male Infertility in DNA Polymerase {lambda}-Deficient Mice: Possible Implication for the Pathogenesis of Immotile Cilia Syndrome Mol. Cell. Biol., April 15, 2002; 22(8): 2769 - 2776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Bertocci, A. De Smet, E. Flatter, A. Dahan, J.-C. Bories, C. Landreau, J.-C. Weill, and C.-A. Reynaud Cutting Edge: DNA Polymerases {micro} and {lambda} Are Dispensable for Ig Gene Hypermutation J. Immunol., April 15, 2002; 168(8): 3702 - 3706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Garcia-Diaz, K. Bebenek, R. Sabariegos, O. Dominguez, J. Rodriguez, T. Kirchhoff, E. Garcia-Palomero, A. J. Picher, R. Juarez, J. F. Ruiz, et al. DNA Polymerase lambda , a Novel DNA Repair Enzyme in Human Cells J. Biol. Chem., April 5, 2002; 277(15): 13184 - 13191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Mizushina, S. Kamisuki, N. Kasai, N. Shimazaki, M. Takemura, H. Asahara, S. Linn, S. Yoshida, A. Matsukage, O. Koiwai, et al. A Plant Phytotoxin, Solanapyrone A, Is an Inhibitor of DNA Polymerase beta and lambda J. Biol. Chem., January 4, 2002; 277(1): 630 - 638. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Zhang, X. Wu, F. Yuan, Z. Xie, and Z. Wang Highly Frequent Frameshift DNA Synthesis by Human DNA Polymerase {micro} Mol. Cell. Biol., December 1, 2001; 21(23): 7995 - 8006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. J. Burgers, E. V. Koonin, E. Bruford, L. Blanco, K. C. Burtis, M. F. Christman, W. C. Copeland, E. C. Friedberg, F. Hanaoka, D. C. Hinkle, et al. Eukaryotic DNA Polymerases: Proposal for a Revised Nomenclature J. Biol. Chem., November 16, 2001; 276(47): 43487 - 43490. [Full Text] [PDF]
|
|
|
|
|
|
M. D. Sutton and G. C. Walker Managing DNA polymerases: Coordinating DNA replication, DNA repair, and DNA recombination PNAS, July 17, 2001; 98(15): 8342 - 8349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. RADMAN, F. TADDEI, and I. MATIC DNA Repair Systems and Bacterial Evolution Cold Spring Harb Symp Quant Biol, January 1, 2000; 65(0): 11 - 20. [Abstract] [PDF]
|
|
|
|
|
|
A. Vaisman, A. Tissier, E. G. Frank, M. F. Goodman, and R. Woodgate Human DNA Polymerase iota Promiscuous Mismatch Extension J. Biol. Chem., August 10, 2001; 276(33): 30615 - 30622. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Garcia-Diaz, K. Bebenek, T. A. Kunkel, and L. Blanco Identification of an Intrinsic 5'-Deoxyribose-5-phosphate Lyase Activity in Human DNA Polymerase lambda . A POSSIBLE ROLE IN BASE EXCISION REPAIR J. Biol. Chem., September 7, 2001; 276(37): 34659 - 34663. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Livneh DNA Damage Control by Novel DNA Polymerases: Translesion Replication and Mutagenesis J. Biol. Chem., July 6, 2001; 276(28): 25639 - 25642. [Full Text] [PDF]
|
|