Nucleic Acids Research, 2000, Vol. 28, No. 19 3762-3770
© 2000 Oxford University Press
CArG binding factor A (CBF-A) is involved in transcriptional regulation of the rat Ha-ras promoter
Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Mailstop C1-015, PO Box 19024, Seattle, WA 98104-2092, USA and 1Department of Microbiology and 2Department of Molecular Biotechnology, University of Washington, Seattle, WA, USA
In the present study we identified a positive transcriptional element within the rat Ha-ras promoter previously known as Ha-ras response element (HRE) and identified a trans-acting factor that binds HRE sequences in rat mammary cells. To identify the binding protein we employed sequence specific DNA affinity chromatography. Amino acid sequence analysis of the affinity-purified proteins was performed by tandem mass spectroscopy. The results unexpectedly demonstrated that in rat mammary cells CArG box-binding factor A (CBF-A) is the major protein species that bind specifically to the rat and human HRE sequences with high affinity. The affinity of CBF-A binding to HRE was significantly higher than to the CArG box described as a recognition sequence for CBF-A protein. Transient transfection assays using reporter plasmids verified that mutations within the HRE that disrupt binding of CBF-A also reduced the activity of the rat Ha-ras promoter. Despite the fact that the HRE within the Ha-ras promoter resembles a binding site for Ets transcription factors, we did not detect the binding of Ets-related proteins to the rat HRE in BICR-M1Rk cells. We further demonstrated a correlation between the presence of HRE binding activity and induction of Ha-ras mRNA expression following serum stimulation in the mammary carcinoma cell line. Taken together, our results suggest that CBF-A may play an important role in transcriptional regulation of Ha-ras promoter activity during normal mammary cell growth and carcinogenesis.
* To whom correspondence should be addressed. Tel: +1 206 667 4107; Fax: +1 206 667 5815; Email: hzarbl@fhcrc.org Present address: Yanni Zhang, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Mailstop C1-015, PO Box 19024, Seattle, WA 98109, USA
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. S. Raju, C. Goritz, Y. Nord, O. Hermanson, C. Lopez-Iglesias, N. Visa, G. Castelo-Branco, and P. Percipalle In Cultured Oligodendrocytes the A/B-type hnRNP CBF-A Accompanies MBP mRNA Bound to mRNA Trafficking Sequences Mol. Biol. Cell, July 1, 2008; 19(7): 3008 - 3019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Inoue, T. Watanabe, K. Tominaga, K. Tsugawa, K. Nishio, K. P. Takahashi, and K. Kaneda Association of hnRNP S1 proteins with vimentin intermediate filaments in migrating cells J. Cell Sci., May 15, 2005; 118(10): 2303 - 2311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Murgatroyd, A. Wigger, E. Frank, N. Singewald, M. Bunck, F. Holsboer, R. Landgraf, and D. Spengler Impaired Repression at a Vasopressin Promoter Polymorphism Underlies Overexpression of Vasopressin in a Rat Model of Trait Anxiety J. Neurosci., September 1, 2004; 24(35): 7762 - 7770. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Boopathi, N. Lenka, S. K. Prabu, J.-K. Fang, F. Wilkinson, M. Atchison, A. Giallongo, and N. G. Avadhani Regulation of Murine Cytochrome c Oxidase Vb Gene Expression during Myogenesis: YY-1 AND HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN D-LIKE PROTEIN (JKTBP1) RECIPROCALLY REGULATE TRANSCRIPTION ACTIVITY BY PHYSICAL INTERACTION WITH THE BERF-1/ZBP-89 FACTOR J. Biol. Chem., August 20, 2004; 279(34): 35242 - 35254. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Khateb, P. Weisman-Shomer, I. Hershco, L. A. Loeb, and M. Fry Destabilization of tetraplex structures of the fragile X repeat sequence (CGG)n is mediated by homolog-conserved domains in three members of the hnRNP family Nucleic Acids Res., August 9, 2004; 32(14): 4145 - 4154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. T. Shi, G.-Y. Yu, and M. M. C. Lai Multiple Type A/B Heterogeneous Nuclear Ribonucleoproteins (hnRNPs) Can Replace hnRNP A1 in Mouse Hepatitis Virus RNA Synthesis J. Virol., October 1, 2003; 77(19): 10584 - 10593. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Desrivieres, T. Prinz, N. Castro-Palomino Laria, M. Meyer, G. Boehm, U. Bauer, J. Schafer, T. Neumann, C. Shemanko, and B. Groner Comparative Proteomic Analysis of Proliferating and Functionally Differentiated Mammary Epithelial Cells Mol. Cell. Proteomics, October 1, 2003; 2(10): 1039 - 1054. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Umar, T. M. Luider, C. A. Berrevoets, J. A. Grootegoed, and A. O. Brinkmann Proteomic Analysis of Androgen-Regulated Protein Expression in a Mouse Fetal Vas Deferens Cell Line Endocrinology, April 1, 2003; 144(4): 1147 - 1154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Weisman-Shomer, E. Cohen, and M. Fry Distinct domains in the CArG-box binding factor A destabilize tetraplex forms of the fragile X expanded sequence d(CGG)n Nucleic Acids Res., September 1, 2002; 30(17): 3672 - 3681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Percipalle, A. Jonsson, D. Nashchekin, C. Karlsson, T. Bergman, A. Guialis, and B. Daneholt Nuclear actin is associated with a specific subset of hnRNP A/B-type proteins Nucleic Acids Res., April 15, 2002; 30(8): 1725 - 1734. [Abstract] [Full Text] [PDF]
|
|