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Nucleic Acids Research, 2000, Vol. 28, No. 2 534-543
© 2000 Oxford University Press

Oligonucleotide-conjugated beads for transdominant genetic experiments

Michael J. Feldhaus, Michael Lualhati, Karen Cardon, Bruce Roth and Alexander Kamb*

Arcaris Inc., 615 Arapeen Drive, Salt Lake City, UT 84108, USA

Transdominant genetics using expression libraries can identify proteins and peptides that affect cell division. In conjunction with these libraries, oligo­nucleotide-conjugated beads and flow cytometry were used to test a strategy that potentially expands the range of such genetic studies. The experimental approach involved creation of tagged expression libraries, introduction of these libraries into cells, growth of the cultured cells for several generations and recovery on oligonucleotide-conjugated beads of sequences that encode growth-modulatory proteins or peptides. Experiments in Saccharomyces cerevisiae demonstrating the feasibility of the strategy are presented.

* To whom correspondence should be addressed. Tel: +1 801 303 0300; Fax: +1 801 303 0333; Email: feldhaus@arcaris.com


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