Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (650K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (18)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Dalgleish, P.
Right arrow Articles by Sharrocks, A. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dalgleish, P.
Right arrow Articles by Sharrocks, A. D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2000, Vol. 28, No. 2 560-569
© 2000 Oxford University Press

The mechanism of complex formation between Fli-1 and SRF transcription factors

Pamela Dalgleish and Andrew D. Sharrocks*

Department of Biochemistry and Genetics, The Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK

The mechanisms of multicomponent transcription factor complex assembly are currently poorly defined. A paradigm for this type of complex is the ETS-domain transcription factor Elk-1 and the MADS-box transcription factor SRF which form a ternary complex with the c-fos serum response element (SRE). In this study we have analysed how a different ETS-domain transcription factor Fli-1 interacts with SRF to form ternary complexes with this element. Two regions of Fli-1 that are required for ternary complex formation have been identified. These SRF binding motifs are located on either side of the ETS DNA-binding domain. Hydrophobic amino acids within these motifs have been identified that play important roles in binding to SRF and ternary complex formation. By using Fli-1 derivatives with mutations in the N-terminal SRF binding motif, the significance of Fli-1–SRF interactions in recruitment of Fli-1 to the c-fos SRE in vivo has been demonstrated. Collectively our data provide a model of how Fli-1 interacts with SRF that differs significantly from the mechanism used by a different ETS-domain protein, Elk-1.

* To whom correspondence should be addressed at present address: School of Biological Sciences, Stopford Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK. Tel: +44 161 275 5979; Fax: +44 161 275 5082; Email: a.d.sharrocks@man.ac.uk


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. X. Zhang, E. Garcia-Gras, D. R. Wycuff, S. J. Marriot, N. Kadeer, W. Yu, E. N. Olson, D. J. Garry, M. S. Parmacek, and R. J. Schwartz
Identification of Direct Serum-response Factor Gene Targets during Me2SO-induced P19 Cardiac Cell Differentiation
J. Biol. Chem., May 13, 2005; 280(19): 19115 - 19126.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Jackers, G. Szalai, O. Moussa, and D. K. Watson
Ets-dependent Regulation of Target Gene Expression during Megakaryopoiesis
J. Biol. Chem., December 10, 2004; 279(50): 52183 - 52190.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Ano, R. Pereira, M. Pironin, I. Lesault, C. Milley, I. Lebigot, C. T. Quang, and J. Ghysdael
Erythroblast Transformation by FLI-1 Depends upon Its Specific DNA Binding and Transcriptional Activation Properties
J. Biol. Chem., January 23, 2004; 279(4): 2993 - 3002.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S.-H. Yang, E. Vickers, A. Brehm, T. Kouzarides, and A. D. Sharrocks
Temporal Recruitment of the mSin3A-Histone Deacetylase Corepressor Complex to the ETS Domain Transcription Factor Elk-1
Mol. Cell. Biol., April 15, 2001; 21(8): 2802 - 2814.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
A. Verger, E. Buisine, S. Carrere, R. Wintjens, A. Flourens, J. Coll, D. Stehelin, and M. Duterque-Coquillaud
Identification of Amino Acid Residues in the ETS Transcription Factor Erg That Mediate Erg-Jun/Fos-DNA Ternary Complex Formation
J. Biol. Chem., May 11, 2001; 276(20): 17181 - 17189.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.