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Nucleic Acids Research, 2000, Vol. 28, No. 20 4037-4043
© 2000 Oxford University Press

Hammerhead-mediated processing of satellite pDo500 family transcripts from Dolichopoda cave crickets

Andres A. Rojas, Alejandro Vazquez-Tello*, Gerardo Ferbeyre, Federica Venanzetti1, Lutz Bachmann2, Bruno Paquin, Valerio Sbordoni1 and Robert Cedergren

Département de Biochimie, Université de Montréal, C.P. 6128, Succ. Centre-Ville, Montréal, Quebec H3C 3J7, Canada, 1Dipartimento di Biologia, Universita di Roma Tor Vergata, Via Orazio Raimondo, 00173 Roma, Italy and 2Pritzker Laboratory for Molecular Systematics and Evolution, The Field Museum, 1400 South Lake Shore Drive, Chicago, IL 60605-2496, USA

This work reports the discovery and functional characterization of catalytically active hammerhead motifs within satellite DNA of the pDo500 family from several Dolichopoda cave cricket species. We show that in vitro transcribed RNA of some members of this satellite DNA family do self-cleave in vitro. This self-cleavage activity is correlated with the efficient in vivo processing of long primary transcripts into monomer-sized RNA. The high sequence conservation of the satellite pDo500 DNA family among genetically isolated Dolichopoda schiavazzii populations, as well as other Dolichopoda species, along with the fact that satellite members are actively transcribed in vivo suggests that the hammerhead-encoding satellite transcripts are under selective pressure, perhaps because they fulfil an important physiological role or function. Remarkably, this is the third example of hammerhead ribozyme structures associated with transcribed repetitive DNA sequences from animals. The possibility that such an association may not be purely coincidental is discussed.

* To whom correspondence should be addressed at present address: Hôpital Sainte-Justine, Centre de Recherche, 3175 Cote Sainte-Catherine ave., Montréal, Quebec H3T 1C5, Canada. Tel: +1 514 345 4931; Fax: +1 514 345 4801; Email: vazqueza@magellan.umontreal.ca Present addresses: Andres A. Rojas, Mycota Biosciences Inc., 225 President-Kennedy Avenue West, Montréal Quebec H2Z 3Y8, Canada Gerardo Ferbeyre, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA Bruno Paquin, Galileo Genomics, 6700 du Parc avenue, Suite 200, Montréal, Quebec H2V 4H9, Canada The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors Dedicated to the late Robert Cedergren


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