Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (154K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Schramm, G.
Right arrow Articles by Roeder, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schramm, G.
Right arrow Articles by Roeder, T.
Related Collections
Right arrow Nucleic acid amplification
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2000, Vol. 28, No. 22 e96
© 2000 Oxford University Press

A simple and reliable 5'-RACE approach

Guido Schramm, Iris Bruchhaus1 and Thomas Roeder*

Universität Hamburg Zoologisches Institut, Martin-Luther-King-Platz 3, D-20146 Hamburg, Germany and 1Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Straße 74, D-20359 Hamburg, Germany

A novel approach for the amplification of cDNA ends is described. It requires only minimal amounts of material, a simple cDNA synthesis reaction and a single PCR reaction to amplify the desired 5'- or 3'-ends of a certain cDNA of interest. It combines the so called CapFinder approach with solid phase cDNA synthesis, thus almost eliminating background problems usually associated with 5'-RACE protocols. This approach could be used to generate complete 5'-ends of numerous cDNAs using only one cDNA synthesis reaction. In combination with LA PCR, several kilobases of unknown 5'-ends could be amplified. It is easy to perform, quick, inexpensive and reliable, which should enable it to replace most currently used 5'-RACE protocols.

* To whom correspondence should be addressed. Tel: +49 40 42838 3941; Fax: +49 40 42838 3937; Email: roeder@zoologie.uni-hamburg.de


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 CSHL ProtocolsHome page
M. A. Frohman
Cap-Switching RACE
CSH Protocols, May 1, 2006; 2006(14): pdb.prot4133 - pdb.prot4133.
[Full Text]

Home page
 J. Biol. Chem.Home page
H. Takeuchi, J.-H. Chen, D. R. O'Reilly, P. C. Turner, and H. H. Rees
Regulation of Ecdysteroid Signaling: Cloning and Characterization of Ecdysone Oxidase. A NOVEL STEROID OXIDASE FROM THE COTTON LEAFWORM, SPODOPTERA LITTORALIS
J. Biol. Chem., July 13, 2001; 276(29): 26819 - 26828.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.