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Nucleic Acids Research, 2000, Vol. 28, No. 4 862-867
© 2000 Oxford University Press

DNA sequence analysis of the photosynthesis region of Rhodobacter sphaeroides 2.4.1T

M. Choudhary and Samuel Kaplan*

Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston, TX 77030, USA

This paper describes the DNA sequence of the photosynthesis region of Rhodobacter sphaeroides 2.4.1T. The photosynthesis gene cluster is located within a ~73 kb AseI genomic DNA fragment containing the puf, puhA, cycA and puc operons. A total of 65 open reading frames (ORFs) have been identified, of which 61 showed significant similarity to genes/proteins of other organisms while only four did not reveal any significant sequence similarity to any gene/protein sequences in the database. The data were compared with the corresponding genes/ORFs from a different strain of R.sphaeroides and Rhodobacter capsulatus, a close relative of R.sphaeroides. A detailed analysis of the gene organization in the photosynthesis region revealed a similar gene order in both species with some notable differences located to the pucBAC=cycA region. In addition, photosynthesis gene regulatory protein (PpsR, FNR, IHF) binding motifs in upstream sequences of a number of photosynthesis genes have been identified and shown to differ between these two species. The difference in gene organization relative to pucBAC and cycA suggests that this region originated independently of the photosynthesis gene cluster of R.sphaeroides.

* To whom correspondence should be addressed. Tel: +1 713 500 5502; Fax: +1 713 500 5499; Email: skaplan@utmmg.med.uth.tmc.edu


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