Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (179K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Surzhikov, S. A.
Right arrow Articles by Mirzabekov, A. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Surzhikov, S. A.
Right arrow Articles by Mirzabekov, A. D.
Related Collections
Right arrow Nucleic acid structure
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2000, Vol. 28, No. 8 E29-00
© 2000 Oxford University Press

Advanced method for oligonucleotide deprotection

Sergey A. Surzhikov, Edward N. Timofeev, Boris K. Chernov, Julia B. Golova and Andrei D. Mirzabekov*

Joint Human Genome Program, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 117984 Moscow, Russia and Argonne National Laboratory, Argonne, IL 60439, USA

A new procedure for rapid deprotection of synthetic oligodeoxynucleotides has been developed. While all known deprotection methods require purification to remove the residual protective groups (e.g. benzamide) and insoluble silicates, the new procedure based on the use of an ammonia-free reagent mixture allows one to avoid the additional purification steps. The method can be applied to deprotect the oligodeoxynucleotides synthesized by using the standard protected nucleoside phosphoramidites dGiBu, dCBz and dABz.

* To whom correspondence should be addressed at: Biochip Technology Center, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439-4833, USA. Tel: +1 630 252 3161; Fax: +1 630 252 9155; Email: amir@everest.bim.anl.gov


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.