Nucleic Acids Research, 2000, Vol. 28, No. 9 2004-2011
© 2000 Oxford University Press
Fission yeast Hrp1, a chromodomain ATPase, is required for proper chromosome segregation and its overexpression interferes with chromatin condensation
1Department of Molecular Biology and Research Center for Cell Differentiation and 2Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151-742, Republic of Korea and 3Karolinska Institute, Department of Biosciences at Novum, Huddinge, Sweden
Hrp1 of Schizosaccharomyces pombe is a member of the CHD protein family, characterized by a chromodomain, a Myb-like telobox-related DNA-binding domain and a SNF2-related helicase/ATPase domain. CHD proteins are thought to be required for modification of the chromatin structure in transcription, but the exact roles of CHD proteins are not known. Here we examine the sub-cellular localization and biochemical activity of Hrp1 and the phenotypes of hrp1
and Hrp1-overexpressing strains. Fluorescence microscopy revealed that Hrp1 protein is targeted to the nucleus. We found that Hrp1 exhibited DNA-dependent ATPase activity, stimulated by both single- and double-stranded DNA. Overexpression of Hrp1 caused slow cell growth accompanied by defective chromosome condensation in anaphase resulting in a ‘cut’ (cell untimely torn) phenotype and chromosome loss. The hrp1 mutation also caused abnormal anaphase and mini-chromosome loss phenotypes. Electron micrographs demonstrated that aberrantly shaped nucleoli appeared in Hrp1-overexpressing cells. Therefore, these results suggest that Hrp1 may play a role in mitotic chromosome segregation and maintenance of chromatin structure by utilizing the energy from ATP hydrolysis.
* To whom correspondence should be addressed. Tel: +82 2 880 6689; Fax: +82 2 887 6279; Email: sdpark@plaza.snu.ac.kr Present address: Yong Hwan Jin, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors
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