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Nucleic Acids Research, 2000, Vol. 28, No. 9 2012-2017
© 2000 Oxford University Press

Functional characterization of Caenorhabditis elegans DNA topoisomerase III{alpha}

You-Chan Kim, Junho Lee1 and Hyeon-Sook Koo*

Department of Biochemistry and 1Department of Biology, College of Science, Yonsei University, Seoul 120-749, Korea

To investigate the function of a DNA topoisomerase III enzyme in Caenorhabditis elegans, the full-length cDNA of C.elegans DNA topoisomerase III{alpha} was cloned. The deduced amino acid sequence exhibited identities of 48 and 39% with those of human DNA topoisomerase III{alpha} and Saccharomyces cerevisiae DNA topoisomerase III, respectively. The overexpressed polypeptide showed an optimal activity for removing negative DNA supercoils at a relatively high temperature of 52–57°C, which is similar to the optimum temperatures of other eukaryotic DNA topoisomerase III enzymes. When topoisomerase III{alpha} expression was interfered with by a cognate double-stranded RNA injection, pleiotropic phenotypes with abnormalities in germ cell proliferation, oogenesis and embryo­genesis appeared. These phenotypes were well correlated with mRNA expression localized in the meiotic cells of gonad and early embryonic cells.

* To whom correspondence should be addressed. Tel: +82 2 361 2701; Fax: +82 2 362 9897; Email: kooh@bubble.yonsei.ac.kr


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