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Nucleic Acids Research, 2001, Vol. 29, No. 1 173-174
© 2001 Oxford University Press

The RDP-II (Ribosomal Database Project)

Bonnie L. Maidak, James R. Cole, Timothy G. Lilburn*, Charles T. Parker Jr, Paul R. Saxman, Ryan J. Farris, George M. Garrity, Gary J. Olsen1, Thomas M. Schmidt2 and James M. Tiedje2

Center for Microbial Ecology, 540 Plant and Soil Sciences Building, Michigan State University, East Lansing, MI 48824-1325, USA, 1Department of Microbiology, University of Illinois, B-103 C&LSL Building, 601 South Goodwin Avenue, Urbana, IL 61801-3714, USA and 2Department of Microbiology and Molecular Genetics, Michigan State University, 294 Giltner Hall, East Lansing, MI 48824-1101, USA

The Ribosomal Database Project (RDP-II), previously described by Maidak et al. [Nucleic Acids Res. (2000), 28, 173–174], continued during the past year to add new rRNA sequences to the aligned data and to improve the analysis commands. Release 8.0 (June 1, 2000) consisted of 16 277 aligned prokaryotic small subunit (SSU) rRNA sequences while the number of eukaryotic and mitochondrial SSU rRNA sequences in aligned form remained at 2055 and 1503, respectively. The number of prokaryotic SSU rRNA sequences more than doubled from the previous release 14 months earlier, and ~75% are longer than 899 bp. An RDP-II mirror site in Japan is now available (http://wdcm.nig.ac.jp/RDP/html/index.html). RDP-II provides aligned and annotated rRNA sequences, derived phylogenetic trees and taxonomic hierarchies, and analysis services through its WWW server (http://rdp.cme.msu.edu/). Analysis services include rRNA probe checking, approximate phylogenetic placement of user sequences, screening user sequences for possible chimeric rRNA sequences, automated alignment, production of similarity matrices and services to plan and analyze terminal restriction fragment polymorphism experiments. The RDP-II email address for questions and comments has been changed from curator{at}cme.msu.edu to rdpstaff{at}msu.edu.

* To whom correspondence should be addressed. Tel: +1 517 432 4998; Fax: +1 517 353 8957; Email: rdpstaff{at}msu.edu


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Appl. Environ. Microbiol.Home page
S.-O. Jeon, J. Bunge, T. Stoeck, K. J.-A. Barger, S.-H. Hong, and S. S. Epstein
Synthetic statistical approach reveals a high degree of richness of microbial eukaryotes in an anoxic water column.
Appl. Envir. Microbiol., October 1, 2006; 72(10): 6578 - 6583.
[Abstract] [Full Text] [PDF]


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Int. J. Syst. Evol. Microbiol.Home page
S. H. Duncan, R. I. Aminov, K. P. Scott, P. Louis, T. B. Stanton, and H. J. Flint
Proposal of Roseburia faecis sp. nov., Roseburia hominis sp. nov. and Roseburia inulinivorans sp. nov., based on isolates from human faeces.
Int J Syst Evol Microbiol, October 1, 2006; 56(Pt 10): 2437 - 2441.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
Y. Hongoh, P. Deevong, S. Hattori, T. Inoue, S. Noda, N. Noparatnaraporn, T. Kudo, and M. Ohkuma
Phylogenetic Diversity, Localization, and Cell Morphologies of Members of the Candidate Phylum TG3 and a Subphylum in the Phylum Fibrobacteres, Recently Discovered Bacterial Groups Dominant in Termite Guts
Appl. Envir. Microbiol., October 1, 2006; 72(10): 6780 - 6788.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
K. E. Ashelford, N. A. Chuzhanova, J. C. Fry, A. J. Jones, and A. J. Weightman
New Screening Software Shows that Most Recent Large 16S rRNA Gene Clone Libraries Contain Chimeras
Appl. Envir. Microbiol., September 1, 2006; 72(9): 5734 - 5741.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
Y. Nakanishi, K. Murashima, H. Ohara, T. Suzuki, H. Hayashi, M. Sakamoto, T. Fukasawa, H. Kubota, A. Hosono, T. Kono, et al.
Increase in Terminal Restriction Fragments of Bacteroidetes-Derived 16S rRNA Genes after Administration of Short-Chain Fructooligosaccharides
Appl. Envir. Microbiol., September 1, 2006; 72(9): 6271 - 6276.
[Abstract] [Full Text] [PDF]


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Int. J. Syst. Evol. Microbiol.Home page
J. M. Wink, R. M. Kroppenstedt, P. Schumann, G. Seibert, and E. Stackebrandt
Actinoplanes liguriensis sp. nov. and Actinoplanes teichomyceticus sp. nov.
Int J Syst Evol Microbiol, September 1, 2006; 56(Pt 9): 2125 - 2130.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
T. Z. DeSantis, P. Hugenholtz, N. Larsen, M. Rojas, E. L. Brodie, K. Keller, T. Huber, D. Dalevi, P. Hu, and G. L. Andersen
Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB.
Appl. Envir. Microbiol., July 1, 2006; 72(7): 5069 - 5072.
[Abstract] [Full Text] [PDF]


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Int. J. Syst. Evol. Microbiol.Home page
A. Lopez-Cortes, M.-L. Fardeau, G. Fauque, C. Joulian, and B. Ollivier
Reclassification of the sulfate- and nitrate-reducing bacterium Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov.
Int J Syst Evol Microbiol, July 1, 2006; 56(Pt 7): 1495 - 1499.
[Abstract] [Full Text] [PDF]


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Int. J. Syst. Evol. Microbiol.Home page
M. M. Kendall, Y. Liu, M. Sieprawska-Lupa, K. O. Stetter, W. B. Whitman, and D. R. Boone
Methanococcus aeolicus sp. nov., a mesophilic, methanogenic archaeon from shallow and deep marine sediments.
Int J Syst Evol Microbiol, July 1, 2006; 56(Pt 7): 1525 - 1529.
[Abstract] [Full Text] [PDF]


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Int. J. Syst. Evol. Microbiol.Home page
R. Osawa, T. Fujisawa, and R. Pukall
Lactobacillus apodemi sp. nov., a tannase-producing species isolated from wild mouse faeces.
Int J Syst Evol Microbiol, July 1, 2006; 56(Pt 7): 1693 - 1696.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
U. Friedrich and J. Lenke
Improved enumeration of lactic Acid bacteria in mesophilic dairy starter cultures by using multiplex quantitative real-time PCR and flow cytometry-fluorescence in situ hybridization.
Appl. Envir. Microbiol., June 1, 2006; 72(6): 4163 - 4171.
[Abstract] [Full Text] [PDF]


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Int. J. Syst. Evol. Microbiol.Home page
C. Bakermans, H. L. Ayala-del-Rio, M. A. Ponder, T. Vishnivetskaya, D. Gilichinsky, M. F. Thomashow, and J. M. Tiedje
Psychrobacter cryohalolentis sp. nov. and Psychrobacter arcticus sp. nov., isolated from Siberian permafrost
Int J Syst Evol Microbiol, June 1, 2006; 56(6): 1285 - 1291.
[Abstract] [Full Text] [PDF]


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Int. J. Syst. Evol. Microbiol.Home page
T. D. Niederberger, D. K. Gotz, I. R. McDonald, R. S. Ronimus, and H. W. Morgan
Ignisphaera aggregans gen. nov., sp. nov., a novel hyperthermophilic crenarchaeote isolated from hot springs in Rotorua and Tokaanu, New Zealand.
Int J Syst Evol Microbiol, May 1, 2006; 56(Pt 5): 965 - 971.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
A. Behnke, J. Bunge, K. Barger, H.-W. Breiner, V. Alla, and T. Stoeck
Microeukaryote Community Patterns along an O2/H2S Gradient in a Supersulfidic Anoxic Fjord (Framvaren, Norway).
Appl. Envir. Microbiol., May 1, 2006; 72(5): 3626 - 3636.
[Abstract] [Full Text] [PDF]


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Int. J. Syst. Evol. Microbiol.Home page
S. L'Haridon, M. L. Miroshnichenko, N. A. Kostrikina, B. J. Tindall, S. Spring, P. Schumann, E. Stackebrandt, E. A. Bonch-Osmolovskaya, and C. Jeanthon
Vulcanibacillus modesticaldus gen. nov., sp. nov., a strictly anaerobic, nitrate-reducing bacterium from deep-sea hydrothermal vents.
Int J Syst Evol Microbiol, May 1, 2006; 56(Pt 5): 1047 - 1053.
[Abstract] [Full Text] [PDF]


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Int. J. Syst. Evol. Microbiol.Home page
B. Nowlan, M. S. Dodia, S. P. Singh, and B. K. C. Patel
Bacillus okhensis sp. nov., a halotolerant and alkalitolerant bacterium from an Indian saltpan.
Int J Syst Evol Microbiol, May 1, 2006; 56(Pt 5): 1073 - 1077.
[Abstract] [Full Text] [PDF]


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Int. J. Syst. Evol. Microbiol.Home page
H. Biebl, B. J. Tindall, R. Pukall, H. Lunsdorf, M. Allgaier, and I. Wagner-Dobler
Hoeflea phototrophica sp. nov., a novel marine aerobic alphaproteobacterium that forms bacteriochlorophyll a.
Int J Syst Evol Microbiol, April 1, 2006; 56(Pt 4): 821 - 826.
[Abstract] [Full Text] [PDF]


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Int. J. Syst. Evol. Microbiol.Home page
R. Pukall, P. Schumann, C. Schutte, R. Gols, and M. Dicke
Acaricomes phytoseiuli gen. nov., sp. nov., isolated from the predatory mite Phytoseiulus persimilis
Int J Syst Evol Microbiol, February 1, 2006; 56(2): 465 - 469.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
H. Kimura, M. Sugihara, K. Kato, and S. Hanada
Selective Phylogenetic Analysis Targeted at 16S rRNA Genes of Thermophiles and Hyperthermophiles in Deep-Subsurface Geothermal Environments
Appl. Envir. Microbiol., January 1, 2006; 72(1): 21 - 27.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
K. E. Ashelford, N. A. Chuzhanova, J. C. Fry, A. J. Jones, and A. J. Weightman
At Least 1 in 20 16S rRNA Sequence Records Currently Held in Public Repositories Is Estimated To Contain Substantial Anomalies
Appl. Envir. Microbiol., December 1, 2005; 71(12): 7724 - 7736.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
C. Lozupone and R. Knight
UniFrac: a New Phylogenetic Method for Comparing Microbial Communities
Appl. Envir. Microbiol., December 1, 2005; 71(12): 8228 - 8235.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
A. Pozhitkov, B. Chernov, G. Yershov, and P. A. Noble
Evaluation of Gel-Pad Oligonucleotide Microarray Technology by Using Artificial Neural Networks
Appl. Envir. Microbiol., December 1, 2005; 71(12): 8663 - 8676.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
M. J. L. Coolen, E. Post, C. C. Davis, and L. J. Forney
Characterization of Microbial Communities Found in the Human Vagina by Analysis of Terminal Restriction Fragment Length Polymorphisms of 16S rRNA Genes
Appl. Envir. Microbiol., December 1, 2005; 71(12): 8729 - 8737.
[Abstract] [Full Text] [PDF]


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JDRHome page
J.D. Rudney, R. Chen, and G. Zhang
Streptococci Dominate the Diverse Flora within Buccal Cells
Journal of Dental Research, December 1, 2005; 84(12): 1165 - 1171.
[Abstract] [Full Text] [PDF]


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Int. J. Syst. Evol. Microbiol.Home page
B. Gao and R. S. Gupta
Conserved indels in protein sequences that are characteristic of the phylum Actinobacteria
Int J Syst Evol Microbiol, November 1, 2005; 55(6): 2401 - 2412.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
Y. Hongoh, P. Deevong, T. Inoue, S. Moriya, S. Trakulnaleamsai, M. Ohkuma, C. Vongkaluang, N. Noparatnaraporn, and T. Kudo
Intra- and Interspecific Comparisons of Bacterial Diversity and Community Structure Support Coevolution of Gut Microbiota and Termite Host
Appl. Envir. Microbiol., November 1, 2005; 71(11): 6590 - 6599.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
H. A. Vrionis, R. T. Anderson, I. Ortiz-Bernad, K. R. O'Neill, C. T. Resch, A. D. Peacock, R. Dayvault, D. C. White, P. E. Long, and D. R. Lovley
Microbiological and Geochemical Heterogeneity in an In Situ Uranium Bioremediation Field Site
Appl. Envir. Microbiol., October 1, 2005; 71(10): 6308 - 6318.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
H. E. O'Brien, J. L. Parrent, J. A. Jackson, J.-M. Moncalvo, and R. Vilgalys
Fungal Community Analysis by Large-Scale Sequencing of Environmental Samples
Appl. Envir. Microbiol., September 1, 2005; 71(9): 5544 - 5550.
[Abstract] [Full Text] [PDF]


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J. Clin. Microbiol.Home page
P. H. Summanen, B. Durmaz, M.-L. Vaisanen, C. Liu, D. Molitoris, E. Eerola, I. M. Helander, and S. M. Finegold
Porphyromonas somerae sp. nov., a Pathogen Isolated from Humans and Distinct from Porphyromonas levii
J. Clin. Microbiol., September 1, 2005; 43(9): 4455 - 4459.
[Abstract] [Full Text] [PDF]


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GutHome page
E de Madaria, J Martinez, B Lozano, L Sempere, S Benlloch, J Such, F Uceda, R Frances, and M Perez-Mateo
Detection and identification of bacterial DNA in serum from patients with acute pancreatitis
Gut, September 1, 2005; 54(9): 1293 - 1297.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
A. Dhillon, M. Lever, K. G. Lloyd, D. B. Albert, M. L. Sogin, and A. Teske
Methanogen Diversity Evidenced by Molecular Characterization of Methyl Coenzyme M Reductase A (mcrA) Genes in Hydrothermal Sediments of the Guaymas Basin
Appl. Envir. Microbiol., August 1, 2005; 71(8): 4592 - 4601.
[Abstract] [Full Text] [PDF]


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MicrobiologyHome page
E. Griffiths, A. K. Petrich, and R. S. Gupta
Conserved indels in essential proteins that are distinctive characteristics of Chlamydiales and provide novel means for their identification
Microbiology, August 1, 2005; 151(8): 2647 - 2657.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
M. G. Wise and G. R. Siragusa
Quantitative Detection of Clostridium perfringens in the Broiler Fowl Gastrointestinal Tract by Real-Time PCR
Appl. Envir. Microbiol., July 1, 2005; 71(7): 3911 - 3916.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
Y. Kong, J. L. Nielsen, and P. H. Nielsen
Identity and Ecophysiology of Uncultured Actinobacterial Polyphosphate-Accumulating Organisms in Full-Scale Enhanced Biological Phosphorus Removal Plants
Appl. Envir. Microbiol., July 1, 2005; 71(7): 4076 - 4085.
[Abstract] [Full Text] [PDF]


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Int. J. Syst. Evol. Microbiol.Home page
K. P. Nevin, D. E. Holmes, T. L. Woodard, E. S. Hinlein, D. W. Ostendorf, and D. R. Lovley
Geobacter bemidjiensis sp. nov. and Geobacter psychrophilus sp. nov., two novel Fe(III)-reducing subsurface isolates
Int J Syst Evol Microbiol, July 1, 2005; 55(4): 1667 - 1674.
[Abstract] [Full Text] [PDF]


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J. Clin. Microbiol.Home page
L. D. Gray, K. L. Ketring, and Y.-W. Tang
Clinical Use of 16S rRNA Gene Sequencing To Identify Mycoplasma felis and M. gateae Associated with Feline Ulcerative Keratitis
J. Clin. Microbiol., July 1, 2005; 43(7): 3431 - 3434.
[Abstract] [Full Text] [PDF]


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Proc. Natl. Acad. Sci. USAHome page
R. W. Hyman, M. Fukushima, L. Diamond, J. Kumm, L. C. Giudice, and R. W. Davis
Microbes on the human vaginal epithelium
PNAS, May 31, 2005; 102(22): 7952 - 7957.
[Abstract] [Full Text] [PDF]


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Mol Biol EvolHome page
A. C. Frank, C. M. Alsmark, M. Thollesson, and S. G. E. Andersson
Functional Divergence and Horizontal Transfer of Type IV Secretion Systems
Mol. Biol. Evol., May 1, 2005; 22(5): 1325 - 1336.
[Abstract] [Full Text] [PDF]


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J. Clin. Microbiol.Home page
Y. Song, C. Liu, M. Bolanos, J. Lee, M. McTeague, and S. M. Finegold
Evaluation of 16S rRNA Sequencing and Reevaluation of a Short Biochemical Scheme for Identification of Clinically Significant Bacteroides Species
J. Clin. Microbiol., April 1, 2005; 43(4): 1531 - 1537.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
C. From, R. Pukall, P. Schumann, V. Hormazabal, and P. E. Granum
Toxin-Producing Ability among Bacillus spp. Outside the Bacillus cereus Group
Appl. Envir. Microbiol., March 1, 2005; 71(3): 1178 - 1183.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
B. D. Eardly, S. M. Nour, P. van Berkum, and R. K. Selander
Rhizobial 16S rRNA and dnaK Genes: Mosaicism and the Uncertain Phylogenetic Placement of Rhizobium galegae
Appl. Envir. Microbiol., March 1, 2005; 71(3): 1328 - 1335.
[Abstract] [Full Text] [PDF]



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