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Nucleic Acids Research, 2001, Vol. 29, No. 10 2041-2051
© 2001 Oxford University Press

Antisense oligonucleotides selected by hybridisation to scanning arrays are effective reagents in vivo

Muhammad Sohail*, Helfrid Hochegger1, Andrea Klotzbücher2, Rene Le Guellec3, Tim Hunt1 and Edwin M. Southern

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK, 1Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK, 2KTB GmbH, Institut für Molekulare Onkologie, Breisacher Strasse 117, D-79106 Freiburg, Germany and 3Unité de Biologie et Genetique du Development, CNRS UPR 41, Universite Rennes I, Avenue du General Leclerc, 35042 Rennes, France

Transcripts representing mRNAs of three Xenopus cyclins, B1, B4 and B5, were hybridised to arrays of oligonucleotides scanning the first 120 nt of the coding region to assess the ability of the immobilised oligonucleotides to form heteroduplexes with their targets. Oligonucleotides that produced high heteroduplex yield and others that showed little annealing were assayed for their effect on translation of endogenous cyclin mRNAs in Xenopus egg extracts and their ability to promote cleavage of cyclin mRNAs in oocytes by RNase H. Excellent correlation was found between antisense potency and affinity of oligonucleotides for the cyclin transcripts as measured by the array, despite the complexity of the cellular environment.

* To whom correspondence should be addressed. Tel: +44 1865 275224; Fax: +44 1865 275259; Email: msohail{at}bioch.ox.ac.uk The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors


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