Nucleic Acids Research, 2001, Vol. 29, No. 12 2448-2455
© 2001 Oxford University Press
The yeast POP2 gene encodes a nuclease involved in mRNA deadenylation
Centre de Génétique Moléculaire, CNRS, Avenue de la Terrasse, F-91198 Gif sur Yvette, France
The major mRNA degradation pathway involves deadenylation of the target molecule followed by decapping and, finally, 5'
3' exonuclease digestion of the mRNA body. While yeast factors involved in the decapping and exonuclease degradation steps have been identified, the nature of the factor(s) involved in the deadenylation step remained elusive. Database searches for yeast proteins related to the mammalian deadenylase PARN identified the Pop2 protein (Pop2p) as a potential deadenylase. While Pop2p was previously identified as a factor affecting transcription, we identified a non-canonical RNase D sequence signature in its sequence. Analysis of the fate of a reporter mRNA in a pop2 mutant demonstrates that Pop2p is required for efficient mRNA degradation in vivo. Characterisation of mRNA degradation intermediates accumulating in this mutant supports the involvement of Pop2p in mRNA deadenylation in vivo. Similar phenotypes are observed in yeast strains lacking the Ccr4 protein, which is known to be associated with Pop2p. A recombinant Pop2p fragment encompassing the putative catalytic domain degrades poly(A) in vitro demonstrating that Pop2p is a nuclease. We also demonstrate that poly(A) is a better competitor than poly(G) or poly(C) of the Pop2p nuclease activity. Altogether, our study indicates that Pop2p is a nuclease subunit of the yeast deadenylase and suggests that Pop2p homologues in other species may have similar functions.
* To whom correspondence should be addressed. Tel: +33 1 69 82 38 84; Fax: +33 1 69 82 38 77; Email: seraphin{at}cgm.cnrs-gif.fr The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Schwede, L. Ellis, J. Luther, M. Carrington, G. Stoecklin, and C. Clayton A role for Caf1 in mRNA deadenylation and decay in trypanosomes and human cells Nucleic Acids Res., June 1, 2008; 36(10): 3374 - 3388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Berretta, M. Pinskaya, and A. Morillon A cryptic unstable transcript mediates transcriptional trans-silencing of the Ty1 retrotransposon in S. cerevisiae Genes & Dev., March 1, 2008; 22(5): 615 - 626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Funakoshi, Y. Doi, N. Hosoda, N. Uchida, M. Osawa, I. Shimada, M. Tsujimoto, T. Suzuki, T. Katada, and S.-i. Hoshino Mechanism of mRNA deadenylation: evidence for a molecular interplay between translation termination factor eRF3 and mRNA deadenylases Genes & Dev., December 1, 2007; 21(23): 3135 - 3148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Nilsson, N. Henriksson, A. Niedzwiecka, N. A. A. Balatsos, K. Kokkoris, J. Eriksson, and A. Virtanen A Multifunctional RNA Recognition Motif in Poly(A)-specific Ribonuclease with Cap and Poly(A) Binding Properties J. Biol. Chem., November 9, 2007; 282(45): 32902 - 32911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Simon and B. Seraphin A specific role for the C-terminal region of the Poly(A)-binding protein in mRNA decay Nucleic Acids Res., September 25, 2007; 35(18): 6017 - 6028. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Georgiev, M. Sjostrom, and A. Wieslander Binding specificities of the GYF domains from two Saccharomyces cerevisiae Paralogs Protein Eng. Des. Sel., September 4, 2007; (2007) gzm041v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Yao, Y.-C. Chiang, C. Zhang, D. J. Lee, T. M. Laue, and C. L. Denis PAB1 Self-Association Precludes Its Binding to Poly(A), Thereby Accelerating CCR4 Deadenylation In Vivo Mol. Cell. Biol., September 1, 2007; 27(17): 6243 - 6253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. James, E. Landrieux, and M. A. Collart A SAGA-Independent Function of SPT3 Mediates Transcriptional Deregulation in a Mutant of the Ccr4-Not Complex in Saccharomyces cerevisiae Genetics, September 1, 2007; 177(1): 123 - 135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Bonisch, C. Temme, B. Moritz, and E. Wahle Degradation of hsp70 and Other mRNAs in Drosophila via the 5' 3' Pathway and Its Regulation by Heat Shock J. Biol. Chem., July 27, 2007; 282(30): 21818 - 21828. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. H. Beilharz and T. Preiss Widespread use of poly(A) tail length control to accentuate expression of the yeast transcriptome RNA, July 1, 2007; 13(7): 982 - 997. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ohn, Y.-C. Chiang, D. J. Lee, G. Yao, C. Zhang, and C. L. Denis CAF1 plays an important role in mRNA deadenylation separate from its contact to CCR4 Nucleic Acids Res., May 14, 2007; 35(9): 3002 - 3015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Jonstrup, K. R. Andersen, L. B. Van, and D. E. Brodersen The 1.4-A crystal structure of the S. pombe Pop2p deadenylase subunit unveils the configuration of an active enzyme Nucleic Acids Res., May 14, 2007; 35(9): 3153 - 3164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Takahashi, K. Kontani, Y. Araki, and T. Katada Caf1 regulates translocation of ribonucleotide reductase by releasing nucleoplasmic Spd1-Suc22 assembly Nucleic Acids Res., February 28, 2007; 35(4): 1187 - 1197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Robin-Lespinasse, S. Sentis, C. Kolytcheff, M.-C. Rostan, L. Corbo, and M. Le Romancer hCAF1, a new regulator of PRMT1-dependent arginine methylation J. Cell Sci., February 15, 2007; 120(4): 638 - 647. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Goldstrohm, D. J. Seay, B. A. Hook, and M. Wickens PUF Protein-mediated Deadenylation Is Catalyzed by Ccr4p J. Biol. Chem., January 5, 2007; 282(1): 109 - 114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. N. Woolstencroft, T. H. Beilharz, M. A. Cook, T. Preiss, D. Durocher, and M. Tyers Ccr4 contributes to tolerance of replication stress through control of CRT1 mRNA poly(A) tail length J. Cell Sci., December 15, 2006; 119(24): 5178 - 5192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Hilgers, D. Teixeira, and R. Parker Translation-independent inhibition of mRNA deadenylation during stress in Saccharomyces cerevisiae RNA, October 1, 2006; 12(10): 1835 - 1845. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-L. Finoux and B. Seraphin In Vivo Targeting of the Yeast Pop2 Deadenylase Subunit to Reporter Transcripts Induces Their Rapid Degradation and Generates New Decay Intermediates J. Biol. Chem., September 8, 2006; 281(36): 25940 - 25947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jeske, S. Meyer, C. Temme, D. Freudenreich, and E. Wahle Rapid ATP-dependent Deadenylation of nanos mRNA in a Cell-free System from Drosophila Embryos J. Biol. Chem., September 1, 2006; 281(35): 25124 - 25133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Keeling, J. Salas-Marco, L. Z. Osherovich, and D. M. Bedwell Tpa1p Is Part of an mRNP Complex That Influences Translation Termination, mRNA Deadenylation, and mRNA Turnover in Saccharomyces cerevisiae. Mol. Cell. Biol., July 1, 2006; 26(14): 5237 - 5248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Gupta and G. Brewer MicroRNAs: New players in an old game. PNAS, March 14, 2006; 103(11): 3951 - 3952. [Full Text] [PDF]
|
|
|
|
|
|
N. A. A. Balatsos, P. Nilsson, C. Mazza, S. Cusack, and A. Virtanen Inhibition of mRNA Deadenylation by the Nuclear Cap Binding Complex (CBC) J. Biol. Chem., February 17, 2006; 281(7): 4517 - 4522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Milligan, C. Torchet, C. Allmang, T. Shipman, and D. Tollervey A Nuclear Surveillance Pathway for mRNAs with Defective Polyadenylation Mol. Cell. Biol., November 15, 2005; 25(22): 9996 - 10004. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Kuai, B. Das, and F. Sherman A nuclear degradation pathway controls the abundance of normal mRNAs in Saccharomyces cerevisiae PNAS, September 27, 2005; 102(39): 13962 - 13967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Mazzoni, A. Serafini, and C. Falcone The Inactivation of KlNOT4, a Kluyveromyces lactis Gene Encoding a Component of the CCR4-NOT Complex, Reveals New Regulatory Functions Genetics, July 1, 2005; 170(3): 1023 - 1032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. BIANCHIN, F. MAUXION, S. SENTIS, B. SERAPHIN, and L. CORBO Conservation of the deadenylase activity of proteins of the Caf1 family in human RNA, April 1, 2005; 11(4): 487 - 494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Lenssen, N. James, I. Pedruzzi, F. Dubouloz, E. Cameroni, R. Bisig, L. Maillet, M. Werner, J. Roosen, K. Petrovic, et al. The Ccr4-Not Complex Independently Controls both Msn2-Dependent Transcriptional Activation--via a Newly Identified Glc7/Bud14 Type I Protein Phosphatase Module--and TFIID Promoter Distribution Mol. Cell. Biol., January 1, 2005; 25(1): 488 - 498. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Traven, A. Hammet, N. Tenis, C. L. Denis, and J. Heierhorst Ccr4-Not Complex mRNA Deadenylase Activity Contributes to DNA Damage Responses in Saccharomyces cerevisiae Genetics, January 1, 2005; 169(1): 65 - 75. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Berthet, A.-M. Morera, M.-J. Asensio, M.-A. Chauvin, A.-P. Morel, F. Dijoud, J.-P. Magaud, P. Durand, and J.-P. Rouault CCR4-Associated Factor CAF1 Is an Essential Factor for Spermatogenesis Mol. Cell. Biol., July 1, 2004; 24(13): 5808 - 5820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Viswanathan, T. Ohn, Y.-C. Chiang, J. Chen, and C. L. Denis Mouse CAF1 Can Function As a Processive Deadenylase/3'-5'-Exonuclease in Vitro but in Yeast the Deadenylase Function of CAF1 Is Not Required for mRNA Poly(A) Removal J. Biol. Chem., June 4, 2004; 279(23): 23988 - 23995. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Cougot, S. Babajko, and B. Seraphin Cytoplasmic foci are sites of mRNA decay in human cells J. Cell Biol., April 12, 2004; 165(1): 31 - 40. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. B. Clark, P. Viswanathan, G. Quigley, Y.-C. Chiang, J. S. McMahon, G. Yao, J. Chen, A. Nelsbach, and C. L. Denis Systematic Mutagenesis of the Leucine-rich Repeat (LRR) Domain of CCR4 Reveals Specific Sites for Binding to CAF1 and a Separate Critical Role for the LRR in CCR4 Deadenylase Activity J. Biol. Chem., April 2, 2004; 279(14): 13616 - 13623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. G. M. Zwartjes, S. Jayne, D. L. C. van den Berg, and H. T. M. Timmers Repression of Promoter Activity by CNOT2, a Subunit of the Transcription Regulatory Ccr4-Not Complex J. Biol. Chem., March 19, 2004; 279(12): 10848 - 10854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. MILONE, J. WILUSZ, and V. BELLOFATTO Characterization of deadenylation in trypanosome extracts and its inhibition by poly(A)-binding protein Pab1p RNA, March 1, 2004; 10(3): 448 - 457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kshirsagar and R. Parker Identification of Edc3p as an Enhancer of mRNA Decapping in Saccharomyces cerevisiae Genetics, February 1, 2004; 166(2): 729 - 739. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Uchida, S.-i. Hoshino, and T. Katada Identification of a Human Cytoplasmic Poly(A) Nuclease Complex Stimulated by Poly(A)-binding Protein J. Biol. Chem., January 9, 2004; 279(2): 1383 - 1391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. van Dijk, H. Le Hir, and B. Seraphin DcpS can act in the 5'-3' mRNA decay pathway in addition to the 3'-5' pathway PNAS, October 14, 2003; 100(21): 12081 - 12086. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Hosoda, T. Kobayashi, N. Uchida, Y. Funakoshi, Y. Kikuchi, S. Hoshino, and T. Katada Translation Termination Factor eRF3 Mediates mRNA Decay through the Regulation of Deadenylation J. Biol. Chem., October 3, 2003; 278(40): 38287 - 38291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Das, J. S. Butler, and F. Sherman Degradation of Normal mRNA in the Nucleus of Saccharomyces cerevisiae Mol. Cell. Biol., August 15, 2003; 23(16): 5502 - 5515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-P. Morel, S. Sentis, C. Bianchin, M. Le Romancer, L. Jonard, M.-C. Rostan, R. Rimokh, and L. Corbo BTG2 antiproliferative protein interacts with the human CCR4 complex existing in vivo in three cell-cycle-regulated forms J. Cell Sci., July 15, 2003; 116(14): 2929 - 2936. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Viswanathan, J. Chen, Y.-C. Chiang, and C. L. Denis Identification of Multiple RNA Features That Influence CCR4 Deadenylation Activity J. Biol. Chem., April 18, 2003; 278(17): 14949 - 14955. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. STEIGER, A. CARR-SCHMID, D. C. SCHWARTZ, M. KILEDJIAN, and R. PARKER Analysis of recombinant yeast decapping enzyme RNA, February 1, 2003; 9(2): 231 - 238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Deluen, N. James, L. Maillet, M. Molinete, G. Theiler, M. Lemaire, N. Paquet, and M. A. Collart The Ccr4-Not Complex and yTAF1 (yTafII130p/yTafII145p) Show Physical and Functional Interactions Mol. Cell. Biol., October 1, 2002; 22(19): 6735 - 6749. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Decker and R. Parker mRNA decay enzymes: Decappers conserved between yeast and mammals PNAS, October 1, 2002; 99(20): 12512 - 12514. [Full Text] [PDF]
|
|
|
|
|
|
J. Milone, J. Wilusz, and V. Bellofatto Identification of mRNA decapping activities and an ARE-regulated 3' to 5' exonuclease activity in trypanosome extracts Nucleic Acids Res., September 15, 2002; 30(18): 4040 - 4050. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Maillet and M. A. Collart Interaction between Not1p, a Component of the Ccr4-Not Complex, a Global Regulator of Transcription, and Dhh1p, a Putative RNA Helicase J. Biol. Chem., January 18, 2002; 277(4): 2835 - 2842. [Abstract] [Full Text] [PDF]
|
|