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Nucleic Acids Research, 2001, Vol. 29, No. 12 2479-2483
© 2001 Oxford University Press

The RNA-binding specificity of the mouse Dazl protein

Julian P. Venables*, Matteo Ruggiu and Howard J. Cooke

Medical Research Council Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK

DAZ is an RNA-binding protein encoded by a region on the Y chromosome implicated in infertility, and DAZ-like (Dazl) proteins are master regulators of germ line gene expression in all animals. In mice Dazl is only expressed in germ cells and is necessary for meiosis. A dual approach was taken to understand the RNA-binding specificity of the Dazl protein: (i) traditional SELEX and (ii) a novel tri-hybrid screen. Both approaches led to the same conclusion, namely that Dazl binds oligo(U) stretches interspersed by G or C residues. In a directed tri-hybrid assay the strongest interaction was with the consensus (GUn)n. This motif is found in the 5' UTR of CDC25C whose homologue is thought to be the target of Boule, the Dazl homologue in flies. CDC25C 5' UTR also interacted specifically with Dazl in vitro. The tri-hybrid screen retrieved UTRs of known genes that may be physiological substrates of Dazl.

* To whom correspondence should be addressed at present address: Institute for Human Genetics, Ridley Building, Claremont Place, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, UK. Tel: +44 191 222 6827; Fax: +44 191 222 6662; Email: j.venables{at}ncl.ac.uk Present address:Matteo Ruggiu, Laboratory of Molecular Neuro-Oncology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA +AF347115, AJ409460–AJ409505


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