Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (225K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (30)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kankia, B. I.
Right arrow Articles by Bloomfield, V. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kankia, B. I.
Right arrow Articles by Bloomfield, V. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2001, Vol. 29, No. 13 2795-2801
© 2001 Oxford University Press

Hexamminecobalt(III)-induced condensation of calf thymus DNA: circular dichroism and hydration measurements

Besik I. Kankia*, Vitaly Buckin1 and Victor A. Bloomfield2 Department of Pharmaceutical Sciences, University of Nebraska Medical Center 986025, Omaha, NE 68198-6025, USA, 1Department of Chemistry, University College Dublin, Belfield, Dublin 4, Ireland and 2Department of Biochemistry, University of Minnesota, St Paul, MN 55108, USA

The interaction of hexamminecobalt(III), Co(NH3)63+, with 160 and 3000–8000 bp length calf thymus DNA has been investigated by circular dichroism, acoustic and densimetric techniques. The acoustic titration curves of 160 bp DNA revealed three stages of interaction: (i) Co(NH3)63+ binding up to the molar ratio [Co(NH3)63+]/[P] = 0.25, prior to DNA condensation; (ii) a condensation process between [Co(NH3)63+]/[P] = 0.25 and 0.30; and (iii) precipitation after [Co(NH3)63+]/[P] = 0.3. In the case of 3000–8000 bp DNA only two processes were observed: (i) binding up to [Co(NH3)63+]/[P] = 0.3; and (ii) precipitation after this point. In agreement with earlier observations, long DNA aggregates without changes in its B-form circular dichroism spectrum, while short DNA demonstrates a positive B->{Psi} transition after [Co(NH3)63+]/[P] = 0.25. From ultrasonic and densimetric measurements the effects of Co(NH3)63+ binding on volume and compressibility have been obtained. The binding of Co(NH3)63+ to both short and long DNA is characterized by similar changes in volume and compressibility calculated per mole Co(NH3)63+: {Delta}V = 9 cm3 mol–1 and {Delta}{kappa} = 33 x 10–4 cm3 mol–1 bar–1. The positive sign of the parameters indicates dehydration, i.e. water release from Co(NH3)63+ and the atomic groups of DNA. This extent of water displacement would be consistent with the formation of two direct, hydrogen bonded contacts between the cation and the phosphates of DNA.

* To whom correspondence should be addressed. Tel: +1 402 559 5384; Fax: +1 402 559 9543; Email: bkankia{at}unmc.edu


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
R. Marty, C. N. N'soukpoe-Kossi, D. M. Charbonneau, L. Kreplak, and H.-A. Tajmir-Riahi
Structural characterization of cationic lipid-tRNA complexes
Nucleic Acids Res., June 26, 2009; (2009) gkp543v1.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. Marty, C. N. N'soukpoe-Kossi, D. Charbonneau, C. M. Weinert, L. Kreplak, and H.-A. Tajmir-Riahi
Structural analysis of DNA complexation with cationic lipids
Nucleic Acids Res., February 1, 2009; 37(3): 849 - 857.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
X. Li, Y. Peng, and X. Qu
Carbon nanotubes selective destabilization of duplex and triplex DNA and inducing B-A transition in solution
Nucleic Acids Res., August 2, 2006; 34(13): 3670 - 3676.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. A. Ouameur and H.-A. Tajmir-Riahi
Structural Analysis of DNA Interactions with Biogenic Polyamines and Cobalt(III)hexamine Studied by Fourier Transform Infrared and Capillary Electrophoresis
J. Biol. Chem., October 1, 2004; 279(40): 42041 - 42054.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
B. I. Kankia
Mg2+-induced triplex formation of an equimolar mixture of poly(rA) and poly(rU)
Nucleic Acids Res., September 1, 2003; 31(17): 5101 - 5107.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Saminathan, T. Thomas, A. Shirahata, C. K. S. Pillai, and T. J. Thomas
Polyamine structural effects on the induction and stabilization of liquid crystalline DNA: potential applications to DNA packaging, gene therapy and polyamine therapeutics
Nucleic Acids Res., September 1, 2002; 30(17): 3722 - 3731.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.