Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (455K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (50)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Guo, D.
Right arrow Articles by Wang, Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guo, D.
Right arrow Articles by Wang, Z.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2001, Vol. 29, No. 13 2875-2883
© 2001 Oxford University Press

Translesion synthesis by yeast DNA polymerase {zeta} from templates containing lesions of ultraviolet radiation and acetylaminofluorene

Dongyu Guo, Xiaohua Wu, Deepak K. Rajpal, John-Stephen Taylor1 and Zhigang Wang* Graduate Center for Toxicology, 306 Health Sciences Research Building, University of Kentucky, Lexington, KY 40536, USA and 1Department of Chemistry, Washington University, St Louis, MO 63130, USA

In the yeast Saccharomyces cerevisiae, DNA polymerase {zeta} (Pol{zeta}) is required in a major lesion bypass pathway. To help understand the role of Pol{zeta} in lesion bypass, we have performed in vitro biochemical analyses of this polymerase in response to several DNA lesions. Purified yeast Pol{zeta} performed limited translesion synthesis opposite a template TT (6-4) photoproduct, incorporating A or T with similar efficiencies (and less frequently G) opposite the 3' T, and predominantly A opposite the 5' T. Purified yeast Pol{zeta} predominantly incorporated a G opposite an acetylaminofluorene (AAF)-adducted guanine. The lesion, however, significantly inhibited subsequent extension. Furthermore, yeast Pol{zeta} catalyzed extension DNA synthesis from primers annealed opposite the AAF-guanine and the 3' T of the TT (6-4) photoproduct with varying efficiencies. Extension synthesis was more efficient when A or C was opposite the AAF-guanine, and when G was opposite the 3' T of the TT (6-4) photoproduct. In contrast, the 3' T of a cis–syn TT dimer completely blocked purified yeast Pol{zeta}, whereas the 5' T was readily bypassed. These results support the following dual-function model of Pol{zeta}. First, Pol{zeta} catalyzes nucleotide incorporation opposite AAF-guanine and TT (6-4) photoproduct with a limited efficiency. Secondly, more efficient bypass of these lesions may require nucleotide incorporation by other DNA polymerases followed by extension DNA synthesis by Pol{zeta}.

* To whom correspondence should be addressed. Tel: +1 859 323 5784; Fax: +1 859 323 1059; Email: zwang{at}pop.uky.edu


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J. A. Brown, S. A. Newmister, K. A. Fiala, and Z. Suo
Mechanism of double-base lesion bypass catalyzed by a Y-family DNA polymerase
Nucleic Acids Res., July 1, 2008; 36(12): 3867 - 3878.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
X. Zhong, P. Garg, C. M. Stith, S. A. N. McElhinny, G. E. Kissling, P. M. J. Burgers, and T. A. Kunkel
The fidelity of DNA synthesis by yeast DNA polymerase zeta alone and with accessory proteins
Nucleic Acids Res., October 18, 2006; 34(17): 4731 - 4742.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Takahashi, A. Sakamoto, S. Sato, T. Kato, S. Tabata, and A. Tanaka
Roles of Arabidopsis AtREV1 and AtREV7 in Translesion Synthesis
Plant Physiology, June 1, 2005; 138(2): 870 - 881.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
P. E. M. Gibbs, J. McDonald, R. Woodgate, and C. W. Lawrence
The Relative Roles in Vivo of Saccharomyces cerevisiae Pol {eta}, Pol {zeta}, Rev1 Protein and Pol32 in the Bypass and Mutation Induction of an Abasic Site, T-T (6-4) Photoadduct and T-T cis-syn Cyclobutane Dimer
Genetics, February 1, 2005; 169(2): 575 - 582.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. G. Jansen, A. Tsaalbi-Shtylik, P. Langerak, F. Calléja, C. M. Meijers, H. Jacobs, and N. de Wind
The BRCT domain of mammalian Rev1 is involved in regulating DNA translesion synthesis
Nucleic Acids Res., January 13, 2005; 33(1): 356 - 365.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Ogi, P. Kannouche, and A. R. Lehmann
Localisation of human Y-family DNA polymerase {kappa}: relationship to PCNA foci
J. Cell Sci., January 1, 2005; 118(1): 129 - 136.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
R. Kusumoto, C. Masutani, S. Shimmyo, S. Iwai, and F. Hanaoka
DNA binding properties of human DNA polymerase {eta}: implications for fidelity and polymerase switching of translesion synthesis
Genes Cells, December 1, 2004; 9(12): 1139 - 1150.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. Pessoa-Brandao and R. A. Sclafani
CDC7/DBF4 Functions in the Translesion Synthesis Branch of the RAD6 Epistasis Group in Saccharomyces cerevisiae
Genetics, August 1, 2004; 167(4): 1597 - 1610.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
B. Zhao, Z. Xie, H. Shen, and Z. Wang
Role of DNA polymerase {eta} in the bypass of abasic sites in yeast cells
Nucleic Acids Res., July 29, 2004; 32(13): 3984 - 3994.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. Guo, Z. Xie, H. Shen, B. Zhao, and Z. Wang
Translesion synthesis of acetylaminofluorene-dG adducts by DNA polymerase {zeta} is stimulated by yeast Rev1 protein
Nucleic Acids Res., February 11, 2004; 32(3): 1122 - 1130.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. G. Kozmin, Y. I. Pavlov, T. A. Kunkel, and E. Sage
Roles of Saccharomyces cerevisiae DNA polymerases Pol{eta} and Pol{zeta} in response to irradiation by simulated sunlight
Nucleic Acids Res., August 1, 2003; 31(15): 4541 - 4552.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I.-Y. Yang, H. Miller, Z. Wang, E. G. Frank, H. Ohmori, F. Hanaoka, and M. Moriya
Mammalian Translesion DNA Synthesis across an Acrolein-derived Deoxyguanosine Adduct. PARTICIPATION OF DNA POLYMERASE eta IN ERROR-PRONE SYNTHESIS IN HUMAN CELLS
J. Biol. Chem., April 11, 2003; 278(16): 13989 - 13994.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Masuda, M. Ohmae, K. Masuda, and K. Kamiya
Structure and Enzymatic Properties of a Stable Complex of the Human REV1 and REV7 Proteins
J. Biol. Chem., March 28, 2003; 278(14): 12356 - 12360.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E. G. Frank, J. M. Sayer, H. Kroth, E. Ohashi, H. Ohmori, D. M. Jerina, and R. Woodgate
Translesion replication of benzo[a]pyrene and benzo[c]phenanthrene diol epoxide adducts of deoxyadenosine and deoxyguanosine by human DNA polymerase {iota}
Nucleic Acids Res., December 1, 2002; 30(23): 5284 - 5292.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Zhang, X. Wu, D. Guo, O. Rechkoblit, J.-S. Taylor, N. E. Geacintov, and Z. Wang
Lesion Bypass Activities of Human DNA Polymerase {micro}
J. Biol. Chem., November 8, 2002; 277(46): 44582 - 44587.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
O. Rechkoblit, Y. Zhang, D. Guo, Z. Wang, S. Amin, J. Krzeminsky, N. Louneva, and N. E. Geacintov
trans-Lesion Synthesis Past Bulky Benzo[a]pyrene Diol Epoxide N2-dG and N6-dA Lesions Catalyzed by DNA Bypass Polymerases
J. Biol. Chem., August 16, 2002; 277(34): 30488 - 30494.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
P. P. H. Van Sloun, I. Varlet, E. Sonneveld, J. J. W. A. Boei, R. J. Romeijn, J. C. J. Eeken, and N. De Wind
Involvement of Mouse Rev3 in Tolerance of Endogenous and Exogenous DNA Damage
Mol. Cell. Biol., April 1, 2002; 22(7): 2159 - 2169.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Y. Zhang, X. Wu, O. Rechkoblit, N. E. Geacintov, J.-S. Taylor, and Z. Wang
Response of human REV1 to different DNA damage: preferential dCMP insertion opposite the lesion
Nucleic Acids Res., April 1, 2002; 30(7): 1630 - 1638.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Zhang and W. Siede
UV-induced T->C transition at a TT photoproduct site is dependent on Saccharomyces cerevisiae polymerase {eta}in vivo
Nucleic Acids Res., March 1, 2002; 30(5): 1262 - 1267.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Interv.Home page
Z. Wang
DNA DAMAGE-INDUCED MUTAGENESIS : A NOVEL TARGET FOR CANCER PREVENTION
Mol. Interv., December 1, 2001; 1(5): 269 - 281.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.