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Nucleic Acids Research, 2001, Vol. 29, No. 15 3181-3187
© 2001 Oxford University Press

Dynamics of association of origins of DNA replication with the nuclear matrix during the cell cycle

Vera Djeliova, George Russev and Boyka Anachkova*

Institute of Molecular Biology, Bulgarian Academy of Sciences, Akad. G. Bonchev Street, Bl. 21, Sofia 1113, Bulgaria

DNA of replication foci attached to the nuclear matrix was isolated from Chinese hamster ovary cells and human HeLa cells synchronized at different stages of the G1 and S phases of the cell cycle. The abundance of sequences from dihydrofolate reductase ori-ß and the ß-globin replicator was determined in matrix-attached DNA. The results show that matrix-attached DNA isolated from cells in late G1 phase was enriched in origin sequences in comparison with matrix-attached DNA from early G1 phase cells. The concentration of the early firing ori-ß in DNA attached to the matrix decreased in early S phase, while the late firing ß-globin origin remained attached until late S phase. We conclude that replication origins associate with the nuclear matrix in late G1 phase and dissociate after initiation of DNA replication in S phase.

* To whom correspondence should be addressed. Tel: +359 2 739954; Fax: +359 2 723507; Email: bar{at}obzor.bio21.bas.bg


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