Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (159K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (16)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Djeliova, V.
Right arrow Articles by Anachkova, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Djeliova, V.
Right arrow Articles by Anachkova, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2001, Vol. 29, No. 15 3181-3187
© 2001 Oxford University Press

Dynamics of association of origins of DNA replication with the nuclear matrix during the cell cycle

Vera Djeliova, George Russev and Boyka Anachkova*

Institute of Molecular Biology, Bulgarian Academy of Sciences, Akad. G. Bonchev Street, Bl. 21, Sofia 1113, Bulgaria

DNA of replication foci attached to the nuclear matrix was isolated from Chinese hamster ovary cells and human HeLa cells synchronized at different stages of the G1 and S phases of the cell cycle. The abundance of sequences from dihydrofolate reductase ori-ß and the ß-globin replicator was determined in matrix-attached DNA. The results show that matrix-attached DNA isolated from cells in late G1 phase was enriched in origin sequences in comparison with matrix-attached DNA from early G1 phase cells. The concentration of the early firing ori-ß in DNA attached to the matrix decreased in early S phase, while the late firing ß-globin origin remained attached until late S phase. We conclude that replication origins associate with the nuclear matrix in late G1 phase and dissociate after initiation of DNA replication in S phase.

* To whom correspondence should be addressed. Tel: +359 2 739954; Fax: +359 2 723507; Email: bar{at}obzor.bio21.bas.bg


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
J. F.-X. Ainscough, F. A. Rahman, H. Sercombe, A. Sedo, B. Gerlach, and D. Coverley
C-terminal domains deliver the DNA replication factor Ciz1 to the nuclear matrix
J. Cell Sci., January 1, 2007; 120(1): 115 - 124.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L. Wang, C.-M. Lin, S. Brooks, D. Cimbora, M. Groudine, and M. I. Aladjem
The Human {beta}-Globin Replication Initiation Region Consists of Two Modular Independent Replicators
Mol. Cell. Biol., April 15, 2004; 24(8): 3373 - 3386.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Ohta, Y. Tatsumi, M. Fujita, T. Tsurimoto, and C. Obuse
The ORC1 Cycle in Human Cells: II. DYNAMIC CHANGES IN THE HUMAN ORC COMPLEX DURING THE CELL CYCLE
J. Biol. Chem., October 17, 2003; 278(42): 41535 - 41540.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.