Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (357K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (11)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Woerfel, G.
Right arrow Articles by Bindereif, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Woerfel, G.
Right arrow Articles by Bindereif, A.
Related Collections
Right arrow RNA characterisation and manipulation
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2001, Vol. 29, No. 15 3204-3211
© 2001 Oxford University Press

In vitro selection of exonic splicing enhancer sequences: identification of novel CD44 enhancers

Gertrud Woerfel and Albrecht Bindereif*

Institut für Biochemie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 58, D-35392 Giessen, Germany

We have developed an in vitro selection procedure that allows the identification and isolation of functional splicing enhancer sequences from any cDNA. It is based on the enhancement of general splicing activity of a pre-mRNA reporter derived from the Drosophila dsx gene. Short DNase I fragments are cloned into a cassette in the second exon of the reporter construct, replacing the natural dsx enhancer. After splicing and reverse transcription–PCR, fragments are recovered from the mRNA product. Applying this selection to the CD44 gene, which undergoes extensive alternative splicing processes, we have identified several novel exonic enhancers. Two of them, which reside in CD44 variable exon 6, were further characterized by mutational analysis and confirmed to function within their natural CD44 context.

* To whom correspondence should be addressed. Tel: +49 641 99 35 420; Fax: +49 641 99 35 419; Email: albrecht.bindereif{at}chemie.bio.uni-giessen.de


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
E. Buratti, C. Stuani, G. De Prato, and F. E. Baralle
SR protein-mediated inhibition of CFTR exon 9 inclusion: molecular characterization of the intronic splicing silencer
Nucleic Acids Res., July 26, 2007; 35(13): 4359 - 4368.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
D.-Q. Xu and W. Mattox
Identification of a splicing enhancer in MLH1 using COMPARE, a new assay for determination of relative RNA splicing efficiencies
Hum. Mol. Genet., January 15, 2006; 15(2): 329 - 336.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Md. T. Nasim, H. M. Chowdhury, and I. C. Eperon
A double reporter assay for detecting changes in the ratio of spliced and unspliced mRNA in mammalian cells
Nucleic Acids Res., October 15, 2002; 30(20): e109 - e109.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.