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Nucleic Acids Research, 2001, Vol. 29, No. 17 3539-3545
© 2001 Oxford University Press

Transcription activation by targeted recruitment of the RNA polymerase II CTD phosphatase FCP1

Paolo Licciardo, Luca Ruggiero, Luigi Lania and Barbara Majello*

Department of Genetics, General and Molecular Biology, University of Naples ‘Federico II’ and IIGB, CNR, Naples, Italy

Human FCP1 in association with RNAP II reconstitutes a highly specific CTD phosphatase activity and is required for recycling RNA polymerase II (RNAP II) in vitro. Here we demonstrate that targeted recruitment of FCP1 to promoter templates, through fusion to a DNA-binding domain, stimulates transcription. We demonstrate that a short region at the C-terminus of the FCP1 protein is required and sufficient for activation, indicating that neither the N-terminal phosphatase domain nor the BRCT domains are required for transcription activity of DNA-bound FCP1. In addition, we demonstrate that the C-terminus region of FCP1 suffices for efficient binding in vivo to the RAP74 subunit of TFIIF and is also required for the exclusive nuclear localization of the protein. These findings suggest a role for FCP1 as a positive regulator of RNAP II transcription.

* To whom correspondence should be addressed at: Department of Genetics, General and Molecular Biology, University of Naples ‘Federico II’, Via Mezzocannone 8, 80134 Naples, Italy. Tel: +39 081 7257257; Fax: +39 081 5936123; Email: majello{at}iigbna.iigb.na.cnr.it


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