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Nucleic Acids Research, 2001, Vol. 29, No. 17 3576-3582
© 2001 Oxford University Press

Completion of RNA synthesis by viral RNA replicases

Robert Tayon Jr, Min-Ju Kim and C. Cheng Kao*

Department of Biology, Indiana University, Bloomington, IN 47405, USA

How the 5'-terminus of the template affects RNA synthesis by viral RNA replicases is poorly understood. Using short DNA, RNA and RNA–DNA chimeric templates that can direct synthesis of replicase products, we found that DNA templates tend to direct the synthesis of RNA products that are shorter by 1 nt in comparison to RNA templates. Template-length RNA synthesis was also affected by the concentration of nucleoside triphosphates, the identity of the bases at specific positions close to the 5'-terminus and the C2'-hydroxyl of a ribose at the third nucleotide from the 5'-terminal nucleotide. Similar requirements are observed with two bromoviral replicases, but not with a recombinant RNA-dependent RNA polymerase. These results begin to define the interactions needed for the viral replicase to complete synthesis of viral RNA.

* To whom correspondence should be addressed. Tel: +1 812 855 7959; Fax: +1 812 855 6705; Email: ckao{at}bio.indiana.edu


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