Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (295K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (20)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Gensler, S.
Right arrow Articles by Wiesner, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gensler, S.
Right arrow Articles by Wiesner, R. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2001, Vol. 29, No. 17 3657-3663
© 2001 Oxford University Press

Mechanism of mammalian mitochondrial DNA replication: import of mitochondrial transcription factor A into isolated mitochondria stimulates 7S DNA synthesis

Sven Gensler1, Katharina Weber2, Wolfgang E. Schmitt1, Acisclo Pérez-Martos3, J. Antonio Enriquez3, Julio Montoya3 and Rudolf J. Wiesner1,2,*

1Department of Physiology II, University of Heidelberg, Heidelberg, Germany, 2Institute of Vegetative Physiology, University of Köln, Robert-Koch-Straße 39, 50931 Köln, Germany and 3Department of Biochemistry and Molecular and Cellular Biology, University of Zaragoza, Spain

The light strand promoter of mammalian mitochondrial DNA gives rise to a primary transcript, but also to the RNA primer necessary for initiation of replication and 7S DNA synthesis as well as 7S RNA. Here we have studied the turnover of 7S DNA in isolated rat liver mitochondria and whether import of mitochondrial transcription factor A (mtTFA), which is necessary for transcription initiation, increases its rate of synthesis. 7S DNA was present as two species, probably due to two different sites of RNA–DNA transition. Time course and pulse–chase experiments showed that the half-life of this DNA is ~45 min. Import of mtTFA, produced in vitro, into the mitochondrial matrix in stoichiometric amounts significantly increased the rate of 7S DNA formation. We conclude that isolated rat liver mitochondria faithfully synthesize and degrade 7S DNA and that increased matrix levels of mtTFA are sufficient to increase its rate of synthesis, strongly supporting the hypothesis that this process is transcription primed.

* To whom correspondence should be addressed at: Institute of Vegetative Physiology, University of Köln, Robert-Koch-Straße 39, 50931 Köln, Germany. Tel: +49 221 478 3610; Fax: +49 221 478 3538; Email: rudolf.wiesner{at}uni-koeln.de


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 FASEB J.Home page
J. Kienhofer, D. J. F. Haussler, F. Ruckelshausen, E. Muessig, K. Weber, D. Pimentel, V. Ullrich, A. Burkle, and M. M. Bachschmid
Association of mitochondrial antioxidant enzymes with mitochondrial DNA as integral nucleoid constituents
FASEB J, July 1, 2009; 23(7): 2034 - 2044.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. L. O. Pohjoismaki, S. Wanrooij, A. K. Hyvarinen, S. Goffart, I. J. Holt, J. N. Spelbrink, and H. T. Jacobs
Alterations to the expression level of mitochondrial transcription factor A, TFAM, modify the mode of mitochondrial DNA replication in cultured human cells
Nucleic Acids Res., November 6, 2006; 34(20): 5815 - 5828.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
H. Tyynismaa, H. Sembongi, M. Bokori-Brown, C. Granycome, N. Ashley, J. Poulton, A. Jalanko, J. N. Spelbrink, I. J. Holt, and A. Suomalainen
Twinkle helicase is essential for mtDNA maintenance and regulates mtDNA copy number
Hum. Mol. Genet., December 15, 2004; 13(24): 3219 - 3227.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Maniura-Weber, S. Goffart, H. L. Garstka, J. Montoya, and R. J. Wiesner
Transient overexpression of mitochondrial transcription factor A (TFAM) is sufficient to stimulate mitochondrial DNA transcription, but not sufficient to increase mtDNA copy number in cultured cells
Nucleic Acids Res., November 16, 2004; 32(20): 6015 - 6027.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
S. Goffart, J.-C. von Kleist-Retzow, and R. J. Wiesner
Regulation of mitochondrial proliferation in the heart: power-plant failure contributes to cardiac failure in hypertrophy
Cardiovasc Res, November 1, 2004; 64(2): 198 - 207.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
R. Depping, S. Hagele, K. F. Wagner, R. J. Wiesner, G. Camenisch, R. H. Wenger, and D. M. Katschinski
A Dominant-Negative Isoform of Hypoxia-Inducible Factor-1{alpha} Specifically Expressed in Human Testis
Biol Reprod, July 1, 2004; 71(1): 331 - 339.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
M. I. Ekstrand, M. Falkenberg, A. Rantanen, C. B. Park, M. Gaspari, K. Hultenby, P. Rustin, C. M. Gustafsson, and N.-G. Larsson
Mitochondrial transcription factor A regulates mtDNA copy number in mammals
Hum. Mol. Genet., May 1, 2004; 13(9): 935 - 944.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. L. Garstka, W. E. Schmitt, J. Schultz, B. Sogl, B. Silakowski, A. Perez-Martos, J. Montoya, and R. J. Wiesner
Import of mitochondrial transcription factor A (TFAM) into rat liver mitochondria stimulates transcription of mitochondrial DNA
Nucleic Acids Res., September 1, 2003; 31(17): 5039 - 5047.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
B. L. Seidel-Rogol and G. S. Shadel
Modulation of mitochondrial transcription in response to mtDNA depletion and repletion in HeLa cells
Nucleic Acids Res., May 1, 2002; 30(9): 1929 - 1934.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. A. Brown and D. A. Clayton
Release of replication termination controls mitochondrial DNA copy number after depletion with 2',3'-dideoxycytidine
Nucleic Acids Res., May 1, 2002; 30(9): 2004 - 2010.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.