Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (292K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Ramírez-Santos, J.
Right arrow Articles by Gómez-Eichelmann, M. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ramírez-Santos, J.
Right arrow Articles by Gómez-Eichelmann, M. C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2001, Vol. 29, No. 2 380-386
© 2001 Oxford University Press

Conserved regulatory elements of the promoter sequence of the gene rpoH of enteric bacteria

Jesús Ramírez-Santos, Julio Collado-Vides1, Martin García-Varela2 and M. Carmen Gómez-Eichelmann*

Departamento de Biología Molecular, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, PO Box 70-228, 04510 México, D. F., México, 1Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, PO Box 565-A, 62100 Cuernavaca Morelos, México and 2Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, PO Box 70-228, 04510 México, D. F., México

The rpoH regulatory region of different members of the enteric bacteria family was sequenced or downloaded from GenBank and compared. In addition, the transcriptional start sites of rpoH of Yersinia frederiksenii and Proteus mirabilis, two distant members of this family, were determined. Sequences similar to the {sigma}70 promoters P1, P4 and P5, to the {sigma}E promoter P3 and to boxes DnaA1, DnaA2, cAMP receptor protein (CRP) boxes CRP1, CRP2 and box CytR present in Escherichia coli K12, were identified in sequences of closely related bacteria such as: E.coli, Shigella flexneri, Salmonella enterica serovar Typhimurium, Citrobacter freundii, Enterobacter cloacae and Klebsiella pneumoniae. In more distant bacteria, Y.frederiksenii and P.mirabilis, the rpoH regulatory region has a distal P1-like {sigma}70 promoter and two proximal promoters: a heat-induced {sigma}E-like promoter and a {sigma}70 promoter. Sequences similar to the regulatory boxes were not identified in these bacteria. This study suggests that the general pattern of transcription of the rpoH gene in enteric bacteria includes a distal {sigma}70 promoter, >200 nt upstream of the initiation codon, and two proximal promoters: a heat-induced {sigma}E-like promoter and a {sigma}70 promoter. A second proximal {sigma}70 promoter under catabolite-regulation is probably present only in bacteria closely related to E.coli.

* To whom correspondence should be addressed. Tel: + 52 5 622 38 52; Fax: +52 5 622 38 91; Email: cargom{at}servidor.unam.mx


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
L. Slamti, J. Livny, and M. K. Waldor
Global Gene Expression and Phenotypic Analysis of a Vibrio cholerae rpoH Deletion Mutant
J. Bacteriol., January 15, 2007; 189(2): 351 - 362.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
A. Janaszak, W. Majczak, B. Nadratowska, A. Szalewska-Palasz, G. Konopa, and A. Taylor
A {sigma}54-dependent promoter in the regulatory region of the Escherichia coli rpoH gene
Microbiology, January 1, 2007; 153(1): 111 - 123.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
L. Laskos, C. S. Ryan, J. A. M. Fyfe, and J. K. Davies
The RpoH-Mediated Stress Response in Neisseria gonorrhoeae Is Regulated at the Level of Activity
J. Bacteriol., December 15, 2004; 186(24): 8443 - 8452.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.