Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (347K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (17)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Drewett, V.
Right arrow Articles by Shaw, P. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Drewett, V.
Right arrow Articles by Shaw, P. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2001, Vol. 29, No. 2 479-487
© 2001 Oxford University Press

DNA-bound transcription factor complexes analysed by mass-spectrometry: binding of novel proteins to the human c-fos SRE and related sequences

Victoria Drewett, Henrik Molina1, Alan Millar2, Silke Muller, Friedrich von Hesler and Peter E. Shaw*

School of Biomedical Sciences, D Floor, University of Nottingham Medical School, Queen’s Medical Centre, Nottingham NG7 2UH, UK, 1Protana, Staermosegaardsvej 16, DK-5230 Odense M, Denmark and 2micromass UK, Floats Road, Wythenshawe, Manchester M23 9LZ, UK

Transcription factors control eukaryotic polymerase II function by influencing the recruitment of multiprotein complexes to promoters and their subsequent integrated function. The complexity of the functional ‘transcriptosome’ has necessitated biochemical fractionation and subsequent protein sequencing on a grand scale to identify individual components. As a consequence, much is now known of the basal transcription complex. In contrast, less is known about the complexes formed at distal promoter elements. The c-fos SRE, for example, is known to bind Serum Response Factor (SRF) and ternary complex factors such as Elk-1. Their interaction with other factors at the SRE is implied but, to date, none have been identified. Here we describe the use of mass-spectrometric sequencing to identify six proteins, SRF, Elk-1 and four novel proteins, captured on SRE duplexes linked to magnetic beads. This approach is generally applicable to the characterisation of nucleic acid-bound protein complexes and the post-translational modification of their components.

* To whom correspondence should be addressed. Tel: +44 115 970 9362; Fax: +44 115 970 9926; Email: peter.shaw{at}nottingham.ac.uk


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Sci SignalHome page
I. V. Lund, Y. Hu, Y. H. Raol, R. S. Benham, R. Faris, S. J. Russek, and A. R. Brooks-Kayal
BDNF Selectively Regulates GABAA Receptor Transcription by Activation of the JAK/STAT Pathway
Sci. Signal., October 14, 2008; 1(41): ra9 - ra9.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H.-M. Zhang, L. Li, N. Papadopoulou, G. Hodgson, E. Evans, M. Galbraith, M. Dear, S. Vougier, J. Saxton, and P. E. Shaw
Mitogen-induced recruitment of ERK and MSK to SRE promoter complexes by ternary complex factor Elk-1
Nucleic Acids Res., May 1, 2008; 36(8): 2594 - 2607.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Hu, I. V. Lund, M. C. Gravielle, D. H. Farb, A. R. Brooks-Kayal, and S. J. Russek
Surface Expression of GABAA Receptors Is Transcriptionally Controlled by the Interplay of cAMP-response Element-binding Protein and Its Binding Partner Inducible cAMP Early Repressor
J. Biol. Chem., April 4, 2008; 283(14): 9328 - 9340.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. Hegarat, G. M. Cardoso, F. Rusconi, J.-C. Francois, and D. Praseuth
Analytical biochemistry of DNA protein assemblies from crude cell extracts
Nucleic Acids Res., July 26, 2007; 35(13): e92 - e92.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
B. Grondin, M. Lefrancois, M. Tremblay, M. Saint-Denis, A. Haman, K. Waga, A. Bedard, D. G. Tenen, and T. Hoang
c-Jun Homodimers Can Function as a Context-Specific Coactivator
Mol. Cell. Biol., April 15, 2007; 27(8): 2919 - 2933.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Bowen, A. Lapham, E. Phillips, I. Yeung, M. Alter-Koltunoff, B.-Z. Levi, V. H. Perry, D. A. Mann, and C. H. Barton
Characterization of the Murine Nramp1 Promoter: REQUIREMENTS FOR TRANSACTIVATION BY Miz-1
J. Biol. Chem., September 19, 2003; 278(38): 36017 - 36026.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
X. Yang, X. Li, T. W. Prow, L. M. Reece, S. E. Bassett, B. A. Luxon, N. K. Herzog, J. Aronson, R. E. Shope, J. F. Leary, et al.
Immunofluorescence assay and flow-cytometry selection of bead-bound aptamers
Nucleic Acids Res., May 15, 2003; 31(10): e54 - e54.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Drewett, A. Devitt, J. Saxton, N. Portman, P. Greaney, N. Cheong, T. F. Alnemri, E. Alnemri, and P. E. Shaw
Serum Response Factor Cleavage by Caspases 3 and 7 Linked to Apoptosis in Human BJAB Cells
J. Biol. Chem., August 31, 2001; 276(36): 33444 - 33451.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.