Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (936K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Solinas, A.
Right arrow Articles by Brown, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Solinas, A.
Right arrow Articles by Brown, T.
Related Collections
Right arrow Nucleic acid amplification
Right arrow Polymorphism/mutation detection
Right arrow DNA characterisation
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2001, Vol. 29, No. 20 e96
© 2001 Oxford University Press

Duplex Scorpion primers in SNP analysis and FRET applications

Antonio Solinas, Lynda J. Brown1, Catherine McKeen1, John M. Mellor, Jamie Nicol, Nicky Thelwell1 and Tom Brown*

Department of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK and 1Oswel Research Products Ltd, Biological and Medical Sciences Building, University of Southampton, Boldrewood, Bassett Crescent East, Southampton SO16 7PX, UK

Scorpions are fluorogenic PCR primers with a probe element attached at the 5'-end via a PCR stopper. They are used in real-time amplicon-specific detection of PCR products in homogeneous solution. Two different formats are possible, the ‘stem–loop’ format and the ‘duplex’ format. In both cases the probing mechanism is intramolecular. We have shown that duplex Scorpions are efficient probes in real-time PCR. They give a greater fluorescent signal than stem–loop Scorpions due to the vastly increased separation between fluorophore and quencher in the active form. We have demonstrated their use in allelic discrimination at the W1282X locus of the ABCC7 gene and shown that they can be used in assays where fluorescence resonance energy transfer is required.

* To whom correspondence should be addressed. Tel: +44 2380 592974; Fax: +44 2380 592991; Email: tb2{at}soton.ac.uk


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
S. Huang, J. Salituro, N. Tang, K.-C. Luk, J. Hackett Jr, P. Swanson, G. Cloherty, W.-B. Mak, J. Robinson, and K. Abravaya
Thermodynamically modulated partially double-stranded linear DNA probe design for homogeneous real-time PCR
Nucleic Acids Res., August 13, 2007; 35(16): e101 - e101.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
J. A. Tomlinson, I. Barker, and N. Boonham
Faster, Simpler, More-Specific Methods for Improved Molecular Detection of Phytophthora ramorum in the Field
Appl. Envir. Microbiol., June 15, 2007; 73(12): 4040 - 4047.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Yi, W. Zhang, and D. Y. Zhang
Molecular Zipper: a fluorescent probe for real-time isothermal DNA amplification
Nucleic Acids Res., July 5, 2006; 34(11): e81 - e81.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
F. H. Sharkey, I. M. Banat, and R. Marchant
Detection and Quantification of Gene Expression in Environmental Bacteriology
Appl. Envir. Microbiol., July 1, 2004; 70(7): 3795 - 3806.
[Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Giglio, P. T. Monis, and C. P. Saint
Demonstration of preferential binding of SYBR Green I to specific DNA fragments in real-time multiplex PCR
Nucleic Acids Res., November 15, 2003; 31(22): e136 - e136.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Yamane
MagiProbe: a novel fluorescence quenching-based oligonucleotide probe carrying a fluorophore and an intercalator
Nucleic Acids Res., October 1, 2002; 30(19): e97 - e97.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. A. J. Darby, M. Sollogoub, C. McKeen, L. Brown, A. Risitano, N. Brown, C. Barton, T. Brown, and K. R. Fox
High throughput measurement of duplex, triplex and quadruplex melting curves using molecular beacons and a LightCycler
Nucleic Acids Res., May 1, 2002; 30(9): e39 - e39.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.