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Nucleic Acids Research, 2001, Vol. 29, No. 22 4581-4588
© 2001 Oxford University Press

An mRNA degrading complex in Rhodobacter capsulatus

Stephanie Jäger, Oliver Fuhrmann, Claudia Heck, Markus Hebermehl, Emile Schiltz1, Reinhard Rauhut* and Gabriele Klug

Institut für Mikro- und Molekularbiologie, Justus Liebig Universität Giessen, Heinrich-Buff-Ring 26–32, 35392 Giessen, Germany and 1Institut für Organische Chemie und Biochemie, Albert-Ludwigs Universität Freiburg, Albertstraße 21, 79104 Freiburg, Germany

An RNA degrading, high molecular weight complex was purified from Rhodobacter capsulatus. N-terminal sequencing, glycerol-gradient centrifugation, and immunoaffinity purification as well as functional assays were used to determine the physical and biochemical characteristics of the complex. The complex comprises RNase E and two DEAD-box RNA helicases of 74 and 65 kDa, respectively. Most surprisingly, the transcription termination factor Rho is a major, firmly associated component of the degradosome.

* To whom correspondence should be addressed. Tel: +49 641 99 35542; Fax: +49 641 99 35549; Email: reinhard.rauhut{at}mikro.bio.uni-giessen.de


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