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Nucleic Acids Research, 2001, Vol. 29, No. 22 4654-4662
© 2001 Oxford University Press

Control of DNA excision efficiency in Paramecium

Karine Dubrana1 and Laurence Amar1,2,*

1Laboratoire de Génétique Moléculaire, Ecole Normale Supérieure, 46 Rue d’Ulm, 75230 Paris Cedex 05, France and 2UPRES-A 8080, IBAIC, Bat 444, 91405 Orsay Cedex, France

Programmed excision of internal eliminated sequences (IESs) occurs at thousands of sites in ciliate genomes. How this is controlled is largely unknown. Here, we report the characterization of the non-efficiently excised 156{psi}G-11 IES from Paramecium primaurelia strain 156 and that of the efficiently excised 168{psi}G-11 IES, an allelic variant from strain 168. Then, we report a genetic and molecular analysis of IES excision efficiency in F1 progeny derived from interstrain crosses and in F2 homozygous progeny derived from F1 autogamy. IES 168{psi}G-11 excision efficiency was ~100% in all cases. IES 156{psi}G-11 excision efficiency was 19 ± 13% in F1 progeny and 0.6 ± 1.1% in F2 progeny. No trans-excision event between IESs 156{psi}G-11 and 168{psi}G-11 was detected within the F1 progeny. These data demonstrate that the excision efficiency of this IES is variable and controlled by a cis-acting element. This should encompass positions 8 and/or 9 of the right IES end, which display allele differences. Finally, the 30-fold stimulation of IES 156{psi}G-11 excision efficiency within F1 progeny relative to F2 progeny demonstrates that Paramecium IES excision efficiency is sensitive either to a conjugation-specific trans-acting factor provided by the zygotic genome, or to homologous chromosome cross-talk.

* To whom correspondence should be addressed at: UPRES-A 8080, IBAIC, Bat 444, 91405 Orsay Cedex, France. Tel: +33 1 69 15 66 38; Fax: +33 1 69 15 68 03; Email: laurence.amar{at}bc4.u-psud.fr Present address:Karine Dubrana, Département de Biologie Moléculaire, Université de Genève, 30 quai Ernest Ansermet, 1205 Genève, Switzerland


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