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Nucleic Acids Research, 2001, Vol. 29, No. 22 4691-4698
© 2001 Oxford University Press

Characterization of the type IV restriction modification system BspLU11III from Bacillus sp. LU11

Konstantin Lepikhov1,2, Andrei Tchernov2, Ludmila Zheleznaja3, Nicholas Matvienko1,*, Joern Walter2 and Thomas A. Trautner2

1Institute of Protein Research, 142292 Pushchino, Moscow Region, Russia, 2Max-Planck-Institut für Molekulare Genetik, Berlin, Germany and 3Institute of Theoretical and Experimental Biophysics, Pushchino, Russia

We report the characterization and cloning of the genes for an unusual type IV restriction–modification system, BspLU11III, from Bacillus sp. LU11. The system consists of two methyltransferases and one endonuclease, which also possesses methyltransferase activity. The three genes of the restriction–modification system, bsplu11IIIMa, bsplu11IIIMb and bsplu11IIIR, are closely linked and tandemly arranged. The corresponding enzymes recognize the dsDNA sequence 5'-GGGAC-3'/5'-GTCCC-3', with M.BspLU11IIIa modifying the A (underlined) of one strand and M.BspLU11IIIb the inner C (underlined) of the other strand. R.BspLU11III has both endonuclease and adenine-specific methyltransferase activities and is able to protect the DNA against cleavage by itself. In contrast to all type IV restriction–modification systems described so far, which have only one adenine-specific methyltransferase, BspLU11III is the first type IV restriction–modification system that includes two methyltransferases, one of them being cytosine specific.

* To whom correspondence should be addressed. Tel: +7 0967 732661; Fax: +7 0959 240493; Email: nikmatv{at}vega.protres.ru


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