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Nucleic Acids Research, 2001, Vol. 29, No. 24 e120
© 2001 Oxford University Press

FspAI, a unique type II restriction endonuclease that recognizes the octanucleotide sequence 5'-RTGC{downarrow}GCAY-3'

A. Kesminiene, Z. Maneliene1, J. Vitkute1, M. Petrusyte1 and A. Janulaitis1,*

MBI Fermentas, and 1Institute of Biotechnology, Graiciuno 8, 2028 Vilnius, Lithuania

A new type II restriction endonuclease designated FspAI has been partially purified from a Flexibacter species Tv-m21K. FspAI recognizes the octanucleotide sequence 5'-RTGC{downarrow}GCAY-3' and cleaves it in the center generating blunt-ended DNA fragments.

* To whom correspondence should be addressed. Tel: +370 2 602110; Fax: +370 2 602116; Email: janulait{at}ibt.lt


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