Nucleic Acids Research, 2001, Vol. 29, No. 3 589-597
© 2001 Oxford University Press
Plant orthologs of p300/CBP: conservation of a core domain in metazoan p300/CBP acetyltransferase-related proteins
Institute for Molecular Biology, University of Zurich, Winterthurerstraße 190, CH-8057 Zurich, Switzerland
p300 and CBP participate as transcriptional coregulators in the execution of a wide spectrum of cellular gene expression programs controlling cell differentiation, growth and homeostasis. Both proteins act together with sequence-specific transcription factors to modify chromatin structure of target genes via their intrinsic acetyltransferase activity directed towards core histones and some transcription factors. So far, p300-related proteins have been described in animals ranging from Drosophila and Caenorhabditis elegans to humans. In this report, we describe p300/CBP-like polypeptides in the plant Arabidopsis thaliana. Interestingly, homology between animal and plant p300/CBP is largely restricted to a C-terminal segment, about 600 amino acids in length, which encompasses acetyltransferase and E1A-binding domains. We have examined whether this conservation in sequence is paralleled by a conservation in function. The same amino acid residues critical for acetyltransferase activity in human p300 are also critical for the function of one of the plant orthologs. Remarkably, plant proteins bind to the adenovirus E1A protein in a manner recapitulating the binding specificity of mammalian p300/CBP. The striking conservation of an extended segment of p300/CBP suggests that it may constitute a functional entity fulfilling functions that may be essential for all metazoan organisms.
* To whom correspondence should be addressed. Tel: +41 1 635 3160; Fax: +41 1 635 6811; Email: eckner{at}molbio.unizh.ch Present address: Urs Lüthi, ESBATECH AG, Winterthurerstraße 190, CH-8057 Zurich, Switzerland
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  D. M. Bond, E. S. Dennis, B. J. Pogson, and E. J. Finnegan Histone Acetylation, VERNALIZATION INSENSITIVE 3, FLOWERING LOCUS C, and the Vernalization Response Mol Plant, April 20, 2009; (2009) ssp021v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Deng, C. Liu, Y. Pei, X. Deng, L. Niu, and X. Cao Involvement of the Histone Acetyltransferase AtHAC1 in the Regulation of Flowering Time via Repression of FLOWERING LOCUS C in Arabidopsis Plant Physiology, April 1, 2007; 143(4): 1660 - 1668. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Bharti, P. von Koskull-Doring, S. Bharti, P. Kumar, A. Tintschl-Korbitzer, E. Treuter, and L. Nover Tomato Heat Stress Transcription Factor HsfB1 Represents a Novel Type of General Transcription Coactivator with a Histone-Like Motif Interacting with the Plant CREB Binding Protein Ortholog HAC1 PLANT CELL, June 1, 2004; 16(6): 1521 - 1535. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-J. Yang The diverse superfamily of lysine acetyltransferases and their roles in leukemia and other diseases Nucleic Acids Res., February 11, 2004; 32(3): 959 - 976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. L. Chua, L. A. Watson, and J. C. Gray The Transcriptional Enhancer of the Pea Plastocyanin Gene Associates with the Nuclear Matrix and Regulates Gene Expression through Histone Acetylation PLANT CELL, June 1, 2003; 15(6): 1468 - 1479. [Abstract] [Full Text]
|
|
|
|
|
|
S. Lafarge and M.-H. Montane Characterization of Arabidopsis thaliana ortholog of the human breast cancer susceptibility gene 1: AtBRCA1, strongly induced by gamma rays Nucleic Acids Res., February 15, 2003; 31(4): 1148 - 1155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Pandey, A. Muller, C. A. Napoli, D. A. Selinger, C. S. Pikaard, E. J. Richards, J. Bender, D. W. Mount, and R. A. Jorgensen Analysis of histone acetyltransferase and histone deacetylase families of Arabidopsis thaliana suggests functional diversification of chromatin modification among multicellular eukaryotes Nucleic Acids Res., December 1, 2002; 30(23): 5036 - 5055. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Reyes, L. Hennig, and W. Gruissem Chromatin-Remodeling and Memory Factors. New Regulators of Plant Development Plant Physiology, November 1, 2002; 130(3): 1090 - 1101. [Full Text] [PDF]
|
|
|
|
 |
|
 E. Kalkhoven, H. Teunissen, A. Houweling, C. P. Verrijzer, and A. Zantema The PHD Type Zinc Finger Is an Integral Part of the CBP Acetyltransferase Domain Mol. Cell. Biol., April 1, 2002; 22(7): 1961 - 1970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Bordoli, S. Husser, U. Luthi, M. Netsch, H. Osmani, and R. Eckner Functional analysis of the p300 acetyltransferase domain: the PHD finger of p300 but not of CBP is dispensable for enzymatic activity Nucleic Acids Res., November 1, 2001; 29(21): 4462 - 4471. [Abstract] [Full Text] [PDF]
|
|