Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (511K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (77)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Grishin, N. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grishin, N. V.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2001, Vol. 29, No. 3 638-643
© 2001 Oxford University Press

KH domain: one motif, two folds

Nick V. Grishin*

Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9050, USA

The K homology (KH) module is a widespread RNA-binding motif that has been detected by sequence similarity searches in such proteins as heterogeneous nuclear ribonucleoprotein K (hnRNP K) and ribosomal protein S3. Analysis of spatial structures of KH domains in hnRNP K and S3 reveals that they are topologically dissimilar and thus belong to different protein folds. Thus KH motif proteins provide a rare example of protein domains that share significant sequence similarity in the motif regions but possess globally distinct structures. The two distinct topologies might have arisen from an ancestral KH motif protein by N- and C-terminal extensions, or one of the existing topologies may have evolved from the other by extension, displacement and deletion. C-terminal extension (deletion) requires ß-sheet rearrangement through the insertion (removal) of a ß-strand in a manner similar to that observed in serine protease inhibitors serpins. Current analysis offers a new look on how proteins can change fold in the course of evolution.

* Tel: +1 214 648 3386; Fax: +1 214 648 9099; Email: grishin{at}chop.swmed.edu


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J. Grillari, M. Loscher, M. Denegri, K. Lee, K. Fortschegger, F. Eisenhaber, P. Ajuh, A. I. Lamond, H. Katinger, and R. Grillari-Voglauer
Blom7{alpha} Is a Novel Heterogeneous Nuclear Ribonucleoprotein K Homology Domain Protein Involved in Pre-mRNA Splicing That Interacts with SNEVPrp19-Pso4
J. Biol. Chem., October 16, 2009; 284(42): 29193 - 29204.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Tu, X. Zhou, J. E. Tropea, B. P. Austin, D. S. Waugh, D. L. Court, and X. Ji
Structure of ERA in complex with the 3' end of 16S rRNA: Implications for ribosome biogenesis
PNAS, September 1, 2009; 106(35): 14843 - 14848.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Lykke-Andersen, D. E. Brodersen, and T. H. Jensen
Origins and activities of the eukaryotic exosome
J. Cell Sci., May 15, 2009; 122(10): 1487 - 1494.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
E. Vanrobays, A. Leplus, Y. N. Osheim, A. L. Beyer, L. Wacheul, and D. L.J. Lafontaine
TOR regulates the subcellular distribution of DIM2, a KH domain protein required for cotranscriptional ribosome assembly and pre-40S ribosome export
RNA, October 1, 2008; 14(10): 2061 - 2073.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
J. Hwang and M. Inouye
RelA Functionally Suppresses the Growth Defect Caused by a Mutation in the G Domain of the Essential Der Protein
J. Bacteriol., May 1, 2008; 190(9): 3236 - 3243.
[Abstract] [Full Text] [PDF]

Home page
 J HeredHome page
B. Buckner, K. A. Swaggart, C. C. Wong, H. A. Smith, K. M. Aurand, M. J. Scanlon, P. S. Schnable, and D. Janick-Buckner
Expression and Nucleotide Diversity of the Maize RIK Gene
J. Hered., February 28, 2008; (2008) esn013v1.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
S. M. Garrey, D. M. Cass, A. M. Wandler, M. S. Scanlan, and J. A. Berglund
Transposition of two amino acids changes a promiscuous RNA binding protein into a sequence-specific RNA binding protein
RNA, January 1, 2008; 14(1): 78 - 88.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. Suzuki, A. Tatsuguchi, E. Matsumoto, M. Kawazoe, T. Kaminishi, M. Shirouzu, Y. Muto, C. Takemoto, and S. Yokoyama
Structural Characterization of the Ribosome Maturation Protein, RimM
J. Bacteriol., September 1, 2007; 189(17): 6397 - 6406.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Abe, T. Jo, Y. Matsuda, C. Matsunaga, T. Katayama, and T. Ueda
Structure and Function of DnaA N-terminal Domains: SPECIFIC SITES AND MECHANISMS IN INTER-DnaA INTERACTION AND IN DnaB HELICASE LOADING ON oriC
J. Biol. Chem., June 15, 2007; 282(24): 17816 - 17827.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
X.-C. Su, S. Jergic, M. A. Keniry, N. E. Dixon, and G. Otting
Solution structure of Domains IVa and V of the {tau} subunit of Escherichia coli DNA polymerase III and interaction with the {alpha} subunit
Nucleic Acids Res., May 14, 2007; 35(9): 2825 - 2832.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Fenn, Z. Du, J. K. Lee, R. Tjhen, R. M. Stroud, and T. L. James
Crystal structure of the third KH domain of human poly(C)-binding protein-2 in complex with a C-rich strand of human telomeric DNA at 1.6 A resolution
Nucleic Acids Res., April 10, 2007; (2007) gkm139v1.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Y. Niv, D. R. Ripoll, J. A. Vila, A. Liwo, E. S. Vanamee, A. K. Aggarwal, H. Weinstein, and H. A. Scheraga
Topology of Type II REases revisited; structural classes and the common conserved core
Nucleic Acids Res., April 1, 2007; 35(7): 2227 - 2237.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Buchet-Poyau, J. Courchet, H. L. Hir, B. Seraphin, J.-Y. Scoazec, L. Duret, C. Domon-Dell, J.-N. Freund, and M. Billaud
Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA-binding proteins differentially localized to processing bodies
Nucleic Acids Res., February 28, 2007; 35(4): 1289 - 1300.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. X. Du, C. C. Yun, A. Bialkowska, and V. W. Yang
Protein Inhibitor of Activated STAT1 Interacts with and Up-regulates Activities of the Pro-proliferative Transcription Factor Kruppel-like Factor 5
J. Biol. Chem., February 16, 2007; 282(7): 4782 - 4793.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. D. Auweter, F. C. Oberstrass, and F. H.-T. Allain
Sequence-specific binding of single-stranded RNA: is there a code for recognition?
Nucleic Acids Res., October 18, 2006; 34(17): 4943 - 4959.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. P. Muench, L. Xu, S. E. Sedelnikova, and D. W. Rice
The essential GTPase YphC displays a major domain rearrangement associated with nucleotide binding
PNAS, August 15, 2006; 103(33): 12359 - 12364.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F.-X. Marsellach, D. Huertas, and F. Azorin
The Multi-KH Domain Protein of Saccharomyces cerevisiae Scp160p Contributes to the Regulation of Telomeric Silencing
J. Biol. Chem., June 30, 2006; 281(26): 18227 - 18235.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Yoo, X. Wu, C. Egile, R. Li, and J.-L. Guan
Interaction of N-WASP with hnRNPK and Its Role in Filopodia Formation and Cell Spreading
J. Biol. Chem., June 2, 2006; 281(22): 15352 - 15360.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
N. H. CHMIEL, D. C. RIO, and J. A. DOUDNA
Distinct contributions of KH domains to substrate binding affinity of Drosophila P-element somatic inhibitor protein
RNA, February 1, 2006; 12(2): 283 - 291.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Sidiqi, J. A. Wilce, J. P. Vivian, C. J. Porter, A. Barker, P. J. Leedman, and M. C. J. Wilce
Structure and RNA binding of the third KH domain of poly(C)-binding protein 1
Nucleic Acids Res., February 24, 2005; 33(4): 1213 - 1221.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
B. Zhao, C. Schneid, D. Iliev, E.-M. Schmidt, V. Wagner, F. Wollnik, and M. Mittag
The Circadian RNA-Binding Protein CHLAMY 1 Represents a Novel Type Heteromer of RNA Recognition Motif and Lysine Homology Domain-Containing Subunits
Eukaryot. Cell, June 1, 2004; 3(3): 815 - 825.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. B. Arnvig, S. Pennell, B. Gopal, and M. J. Colston
A high-affinity interaction between NusA and the rrn nut site in Mycobacterium tuberculosis
PNAS, June 1, 2004; 101(22): 8325 - 8330.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Donnini, A. Lapucci, L. Papucci, E. Witort, A. Jacquier, G. Brewer, A. Nicolin, S. Capaccioli, and N. Schiavone
Identification of TINO: A NEW EVOLUTIONARILY CONSERVED BCL-2 AU-RICH ELEMENT RNA-BINDING PROTEIN
J. Biol. Chem., May 7, 2004; 279(19): 20154 - 20166.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
E. VANROBAYS, J.-P. GELUGNE, M. CAIZERGUES-FERRER, and D. L.J. LAFONTAINE
Dim2p, a KH-domain protein required for small ribosomal subunit synthesis
RNA, April 1, 2004; 10(4): 645 - 656.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. N. Chkheidze and S. A. Liebhaber
A Novel Set of Nuclear Localization Signals Determine Distributions of the {alpha}CP RNA-Binding Proteins
Mol. Cell. Biol., December 1, 2003; 23(23): 8405 - 8415.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. M. Goolsby and D. J. Shapiro
RNAi-mediated depletion of the 15 KH domain protein, vigilin, induces death of dividing and non-dividing human cells but does not initially inhibit protein synthesis
Nucleic Acids Res., October 1, 2003; 31(19): 5644 - 5653.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. Watanabe, M. Hamano, H. Kakeshita, K. Bunai, S. Tojo, H. Yamaguchi, Y. Fujita, S.-L. Wong, and K. Yamane
Mannitol-1-Phosphate Dehydrogenase (MtlD) Is Required for Mannitol and Glucitol Assimilation in Bacillus subtilis: Possible Cooperation of mtl and gut Operons
J. Bacteriol., August 15, 2003; 185(16): 4816 - 4824.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. Anantharaman, E. V. Koonin, and L. Aravind
Comparative genomics and evolution of proteins involved in RNA metabolism
Nucleic Acids Res., April 1, 2002; 30(7): 1427 - 1464.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.