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Nucleic Acids Research, 2001, Vol. 29, No. 4 e25
© 2001 Oxford University Press

Diversity Arrays: a solid state technology for sequence information independent genotyping

Damian Jaccoud1, Kaiman Peng1,2, David Feinstein3 and Andrzej Kilian1,*

1Center for the Application of the Molecular Biology to International Agriculture (CAMBIA) Canberra, ACT, Australia, 2National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China and 3Analytic Animations, Bend, OR, USA

Here we present the successful application of the microarray technology platform to the analysis of DNA polymorphisms. Using the rice genome as a model, we demonstrate the potential of a high-throughput genome analysis method called Diversity Array Technology, DArT‘. In the format presented here the technology is assaying for the presence (or amount) of a specific DNA fragment in a representation derived from the total genomic DNA of an organism or a population of organisms. Two different approaches are presented: the first involves contrasting two representations on a single array while the second involves contrasting a representation with a reference DNA fragment common to all elements of the array. The Diversity Panels created using this method allow genetic fingerprinting of any organism or group of organisms belonging to the gene pool from which the panel was developed. Diversity Arrays enable rapid and economical application of a highly parallel, solid-state genotyping technology to any genome or complex genomic mixtures.

* To whom correspondence should be addressed. Tel: +61 2 6246 4519; Fax: +61 2 6246 4533; Email: a.kilian@cambia.org The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors


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