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Nucleic Acids Research, 2001, Vol. 29, No. 8 1808-1814
© 2001 Oxford University Press

E2F-dependent transcription of the raf proto-oncogene during Drosophila development

Eun-Jeong Kwon1,2,3, Eun-Jin Oh1, Young-Shin Kim1, Fumiko Hirose2, Katsuhito Ohno2,3, Yasuyoshi Nishida3, Akio Matsukage2, Masamitsu Yamaguchi2 and Mi-Ae Yoo1,*

1Department of Molecular Biology, College of Natural Science, Pusan National University, Pusan 609-735, Korea, 2Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya 464-8681, Japan and 3Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan

D-raf, a Drosophila homolog of the raf proto-oncogene, has diverse functions throughout development and is transcribed in a wide range of tissues, with high levels of expression in the ovary and in association with rapid proliferation. The expression pattern resembles those of S phase genes, which are regulated by E2F transcription factors. In the 5'-flanking region of D-raf, four sequences (E2F sites 1–4) similar to the E2F recognition sequence were found, one of them (E2F site 3) being recognized efficiently by Drosophila E2F (dE2F) in vitro. Transient luciferase expression assays confirmed activation of the D-raf gene promoter by dE2F/dDP. Expression of Draf–lacZ was greatly reduced in embryos homozygous for the dE2F mutation. These results suggest that dE2F is likely to be an important regulator of D-raf transcription.

* To whom correspondence should be addressed. Tel: +82 51 510 2278; Fax: +82 51 513 9258; Email: mayoo{at}hyowon.cc.pusan.ac.kr Correspondence may also be addressed to Masamitsu Yamaguchi. Tel: +81 52 762 6111; Fax: +81 52 763 5233; Email: myamaguc@aichi-cc.pref.aichi.jp Present address: Akio Matsukage, Chemical and Biological Sciences, Faculty of Science, Japan Women’s University, 2-8-1 Mejirodai, Bunkyou-ku, Tokyo 112-8681, Japan The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors


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M.-S. Hwang, Y.-S. Kim, N.-H. Choi, J.-H. Park, E.-J. Oh, E.-J. Kwon, M. Yamaguchi, and M.-A. Yoo
The Caudal homeodomain protein activates Drosophila E2F gene expression
Nucleic Acids Res., December 1, 2002; 30(23): 5029 - 5035.
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