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Nucleic Acids Research, 2001, Vol. 29, No. 9 1898-1905
© 2001 Oxford University Press

Enhancing the catalytic repertoire of nucleic acids. II. Simultaneous incorporation of amino and imidazolyl functionalities by two modified triphosphates during PCR

Thierry Gourlain, Alexander Sidorov, Nathalie Mignet, Simon J. Thorpe, Sarah E. Lee, Jane A Grasby and David M. Williams*

Centre for Chemical Biology, Department of Chemistry, Krebs Institute, University of Sheffield, Sheffield S3 7HF, UK

The incorporation of potentially catalytic groups into DNA is of interest for the in vitro selection of novel deoxyribozymes. We have devised synthetic routes to a series of three C7 modified 7-deaza-dATP derivatives with pendant aminopropyl, Z-aminopropenyl and aminopropynyl side chains. These modified triphosphates have been tested as substrates for Taq polymerase during PCR. All the modifications are tolerated by this enzyme, with the aminopropynyl side chain giving the best result. Most protein enzymes have more than one type of catalytic group located in their active site. By using C5-imidazolyl-modified dUTPs together with 3-(aminopropynyl)-7-deaza-dATP in place of the natural nucleotides dTTP and dATP, we have demonstrated the simultaneous incorporation of both amino and imidazolyl moieties into a DNA molecule during PCR. The PCR product containing the four natural bases was fully digested by XbaI, while PCR products containing the modified 7-deaza-dATP analogues were not cleaved. Direct evidence for the simultaneous incorporation during PCR of an imidazole-modified dUTP and an amino-modified 7-deaza-dATP has been obtained using mass spectrometry.

* To whom correspondence should be addressed. Tel: +44 114 222 9502; Fax: +44 114 273 8673; Email: d.m.williams{at}sheffield.ac.uk Correspondence may also be addressed to Jane A. Grasby. Tel +44 114 222 9502; Fax: +44 114 273 8673; Email: j.a.grasby{at}sheffield.ac.uk Present address: Nathalie Mignet, Aventis Pharma/UMR7001 CNRS-ENSCP, Bat. Monod 3CO1, 13 quai Jules Guesde BP14, 94403 Vitry cedex, France


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