Nucleic Acids Research, 1976, Vol. 3, No. 2 441-448
© 1976
Articles |
A modified uridine in the anticodon of E. coli $${\hbox{ tRNA }}_{\hbox{ 1 }}^{\hbox{ Tyr }}\hbox{ \hspace{0.17em} }{\hbox{ su }}_{\hbox{ oc }}^{+}$$
Department of Biology, Yale University New Haven, CT 06520, USA.
Received December 17, 1975.
The anticodon of an ochre-suppressing derivative of E. coli 
, previously identified as UUA, can contain a modified uridlne (U$) in the first position. The novel modified nudeotide has been identified by twodimensional thin layer chromatography following RNase T2 digestion of anti-codon-containing fragments. 
is found in less than stoichiometric molar yields in preparations of 
. The electrophoretlc mobility of is the same as Up at pH 3.5 and PH 7.5. U$ probably does not contain sulfur since it cannot be labeled with 35S in vivo incorporation experiments.