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Nucleic Acids Research, 2002, Vol. 30, No. 1 230-231
© 2002 Oxford University Press

The DT40 web site: sampling and connecting the genes of a B cell line

Jean-Marie Buerstedde*, Hiroshi Arakawa, Akira Watahiki1, Piere Piero Carninci1, Y. Yoshihide Hayashizaki1, Bernhard Korn2 and Jiri Plachy3

Department of Cellular Immunology, Heinrich-Pette-Institute, Martinistrasse 52, 20251 Hamburg, Germany, 1Laboratory for Genome Exploration Research Project, Genomic Sciences Center and Genome Science Laboratory, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan, Genome Exploration Research Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan, 2RZPD—Ressourcenzentrum für Genomforschung, INF506, 69120 Heidelberg, Germany and 3Academy of Sciences of the Czech Republic, Institut of Molecular Genetics, Flemingovo nam. 2, 16637 Praha 6, Czech Republic

Thousands of new vertebrate genes have been discovered and genetic systems are needed to address their functions at the cellular level. The chicken B cell line DT40 allows efficient gene disruptions due to its high homologous recombination activity. However, cloning the gene of interest is often cumbersome, since relatively few chicken cDNA sequences are present in the public databases. In addition, the accumulation of multiple mutations within the same cell clone is limited by the consumption of one drug-resistance marker for each transfection. Here, we present the DT40 web site (http://genetics.hpi.uni-hamburg.de/dt40.html), which includes a comprehensive database of chicken bursal ESTs to identify disruption candidate genes and recyclable marker cassettes based on the loxP system. These freely available resources greatly facilitate the analysis of genes and genetic networks.

* To whom correspondence should be addressed. Tel: +49 40 48051 290; Fax: +49 40 48051 296; Email: buersted{at}genetics.hpi.uni-hamburg.de


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