Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (579K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Carastro, L. M.
Right arrow Articles by Downey, K. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Carastro, L. M.
Right arrow Articles by Downey, K. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2002, Vol. 30, No. 10 2232-2243
© 2002 Oxford University Press

Identification of delta helicase as the bovine homolog of HUPF1: demonstration of an interaction with the third subunit of DNA polymerase delta

L. Michael Carastro1, Cheng-Keat Tan2, Manuel Selg3, Hans-Martin Jack3, Antero G. So1,2 and Kathleen M. Downey1,2,*

1Department of Biochemistry and Molecular Biology and 2Department of Medicine, University of Miami School of Medicine, Miami, FL 33101, USA and 3Division of Molecular Immunology, Department of Internal Medicine III, Nikolaus-Fiebiger Center, University of Erlangen-Nurnberg, Erlangen, Germany

Delta helicase is a 5' to 3' DNA helicase that partially co-purifies with DNA polymerase delta (pol delta) from fetal bovine thymus tissue. We describe the resolution of delta helicase from pol delta on heparin–agarose chromatography and its purification to apparent homogeneity by affinity purification on single-stranded DNA–cellulose chromatography, unique-sequence RNA–agarose chromatography, and ceramic hydroxyapatite chromatography. Delta helicase isolated from fetal bovine thymus had an apparent Mr of 115 kDa in SDS–PAGE, and photo-crosslinked to [{alpha}-32P]ATP. Tandem mass spectrometry peptide mass data derived from the bovine polypeptide matched to human UPF1 (HUPF1), a 5' to 3' RNA and DNA helicase, and a requisite component of the mRNA surveillance complex. Antisera against HUPF1 cross-reacted with delta helicase on western analysis, and delta helicase activity was immunoinactivated by pre-incubation with antibodies to HUPF1, suggesting that delta helicase is the bovine homolog of HUPF1. Immunoprecipitation experiments demonstrated that HUPF1 interacts with the 66-kDa third subunit of pol delta in vivo.

* To whom correspondence should be addressed at: Department of Medicine (R-99), University of Miami School of Medicine, PO Box 016960, Miami, FL 33101, USA. Tel: +1 305 243 6304; Fax: +1 305 243 4519; Email: kdowney{at}med.miami.edu Present address:L. Michael Carastro, The Salk Institute for Biological Studies, Gene Expression Laboratories, La Jolla, CA 92037, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
B. Luke, C. M. Azzalin, N. Hug, A. Deplazes, M. Peter, and J. Lingner
Saccharomyces cerevisiae Ebs1p is a putative ortholog of human Smg7 and promotes nonsense-mediated mRNA decay
Nucleic Acids Res., December 3, 2007; 35(22): 7688 - 7697.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Wittmann, E. M. Hol, and H.-M. Jack
hUPF2 Silencing Identifies Physiologic Substrates of Mammalian Nonsense-Mediated mRNA Decay
Mol. Cell. Biol., February 15, 2006; 26(4): 1272 - 1287.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
L. E. Maquat
Nonsense-mediated mRNA decay in mammals
J. Cell Sci., May 1, 2005; 118(9): 1773 - 1776.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Lu, C.-K. Tan, J.-Q. Zhou, M. You, L. M. Carastro, K. M. Downey, and A. G. So
Direct Interaction of Proliferating Cell Nuclear Antigen with the Small Subunit of DNA Polymerase delta
J. Biol. Chem., June 28, 2002; 277(27): 24340 - 24345.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.