Nucleic Acids Research, 2002, Vol. 30, No. 11 2270-2279
© 2002 Oxford University Press
Synergistic activation of the rat laminin 
1 chain promoter by the gut-enriched Kruppel-like factor (GKLF/KLF4) and Sp1
Department of Medicine, Box 356521, University of Washington, Seattle, WA 98195, USA
Laminin is a multifunctional heterotrimeric protein present in extracellular matrix where it regulates processes that compose tissue architecture including cell differentiation. Laminin 
1 is the most widely expressed laminin chain and its absence causes early lethality in mouse embryos. Laminin 1 chain gene (LAMC1) promoter contains several GC/GT-rich motifs including the bcn-1 element. Using the bcn-1 element as a bait in the yeast one-hybrid screen, we cloned the gut-enriched Kruppel-like factor (GKLF or KLF4) from a rat mesangial cell library. We show that GKLF binds bcn-1, but this binding is not required for the GKLF-mediated activation of the LAMC1 promoter. The activity of GKLF is dependent on a synergism with another Kruppel-like factor, Sp1. The LAMC1 promoter appears to have multiple GKLF- and Sp1-responsive elements which may account for the synergistic activation. We provide evidence that the synergistic action of GKLF and Sp1 is dependent on the promoter context and the integrity of GKLF activation and DNA-binding domain. GKLF is thought to participate in the switch from cell proliferation to differentiation. Thus, the Sp1–GKLF synergistic activation of the LAMC1 promoter may be one of the avenues for expression of laminin 1 chain when laminin is needed to regulate cell differentiation.
* To whom correspondence should be addressed. Tel: +1 206 543 3792; Fax: +1 206 685 8661; Email: karolb{at}u.washington.edu
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. D. Nelson, S. Flanagin, Y. Kawata, O. Denisenko, and K. Bomsztyk Transcription of laminin {gamma}1 chain gene in rat mesangial cells: constitutive and inducible RNA polymerase II recruitment and chromatin states Am J Physiol Renal Physiol, March 1, 2008; 294(3): F525 - F533. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Ai, H. Zheng, X. Yang, Y. Liu, and T. C. Wang Tip60 functions as a potential corepressor of KLF4 in regulation of HDC promoter activity Nucleic Acids Res., September 25, 2007; 35(18): 6137 - 6149. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Wei, M. Kanai, S. Huang, and K. Xie Emerging role of KLF4 in human gastrointestinal cancer Carcinogenesis, January 1, 2006; 27(1): 23 - 31. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Bhattacharya, S. SenBanerjee, Z. Lin, S. Mir, A. Hamik, P. Wang, P. Mukherjee, D. Mukhopadhyay, and M. K. Jain Inhibition of Vascular Permeability Factor/Vascular Endothelial Growth Factor-mediated Angiogenesis by the Kruppel-like Factor KLF2 J. Biol. Chem., August 12, 2005; 280(32): 28848 - 28851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Noti, A. K. Johnson, and J. D. Dillon The Leukocyte Integrin Gene CD11d Is Repressed by Gut-enriched Kruppel-like Factor 4 in Myeloid Cells J. Biol. Chem., February 4, 2005; 280(5): 3449 - 3457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. SenBanerjee, Z. Lin, G. B. Atkins, D. M. Greif, R. M. Rao, A. Kumar, M. W. Feinberg, Z. Chen, D. I. Simon, F. W. Luscinskas, et al. KLF2 Is a Novel Transcriptional Regulator of Endothelial Proinflammatory Activation J. Exp. Med., May 17, 2004; 199(10): 1305 - 1315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Ai, Y. Liu, M. Langlois, and T. C. Wang Kruppel-like Factor 4 (KLF4) Represses Histidine Decarboxylase Gene Expression through an Upstream Sp1 Site and Downstream Gastrin Responsive Elements J. Biol. Chem., March 5, 2004; 279(10): 8684 - 8693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Piccinni, A.-L. Bolcato-Bellemin, A. Klein, V. W. Yang, M. Kedinger, P. Simon-Assmann, and O. Lefebvre Kruppel-like Factors Regulate the Lama1 Gene Encoding the Laminin {alpha}1 Chain J. Biol. Chem., March 5, 2004; 279(10): 9103 - 9114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. F. Hinnebusch, A. Siddique, J. W. Henderson, M. S. Malo, W. Zhang, C. P. Athaide, M. A. Abedrapo, X. Chen, V. W. Yang, and R. A. Hodin Enterocyte differentiation marker intestinal alkaline phosphatase is a target gene of the gut-enriched Kruppel-like factor Am J Physiol Gastrointest Liver Physiol, January 1, 2004; 286(1): G23 - G30. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Niimi, Y. Hayashi, and K. Sekiguchi Identification of an Upstream Enhancer in the Mouse Laminin alpha 1 Gene Defining Its High Level of Expression in Parietal Endoderm Cells J. Biol. Chem., March 7, 2003; 278(11): 9332 - 9338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. W. Schnaper, T. Hayashida, S. C. Hubchak, and A.-C. Poncelet TGF-beta signal transduction and mesangial cell fibrogenesis Am J Physiol Renal Physiol, February 1, 2003; 284(2): F243 - F252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yasuda, K. Hirayoshi, H. Hirata, H. Kubota, N. Hosokawa, and K. Nagata The Kruppel-like Factor Zf9 and Proteins in the Sp1 Family Regulate the Expression of HSP47, a Collagen-specific Molecular Chaperone J. Biol. Chem., November 15, 2002; 277(47): 44613 - 44622. [Abstract] [Full Text] [PDF]
|
|