Nucleic Acids Research, 2002, Vol. 30, No. 11 2417-2426
© 2002 Oxford University Press
Distinct proteins encoded by alternative transcripts of the PURG gene, located contrapodal to WRN on chromosome 8, determined by differential termination/polyadenylation
Departments of Pathology and Molecular Biology and the D. H. Ruttenberg Cancer Center, Box 1194, Mount Sinai School of Medicine, New York, NY 10029, USA
A gene encoding a new member of the Pur protein family, Pur
, has been detected upstream of, and contrapodal to, the gene encoding the Werner syndrome helicase, Wrn, at human chromosome band 8p11–12. Both the PURG and WRN genes initiate transcription at multiple sites, the major clusters of which are 
90 bp apart. A segment containing this region strongly promotes transcription of a reporter gene in both directions. Both promoters are TATA-less and CAAT-less and both are positively regulated by Sp1 elements. While promoter elements for the two genes are interleaved, in the contrapodal direction, certain elements critical for each gene are distinct. Sequencing of cDNAs for Pur mRNA reveals that two alternative coding sequences are generated from a single gene, resulting in different Pur C-termini. PURG-A mRNA consists of a single intronless transcript of 3 kb. PURG-B mRNA results from transcription through the PURG-A polyadenylation site and splicing out of an intron of >30 kb. In this unique example of a switch, splicing of a single intron either occurs or does not occur depending upon differential termination/polyadenylation. PURG-B is the primary PURG transcript detected in testis, but it is undetectable in all members of a normal adult tissue cDNA panel. PURG-A levels are low or undetectable in the normal tissue panel, but they are greatly elevated in all members of a tumor tissue panel. PURG-B is detected in several tumor panel members.
* To whom correspondence should be addressed. Tel: +1 212 241 7510; Fax: +1 212 534 7491; Email: edward.johnson{at}mssm.edu +AF195513.2, AV077841, AF479672, AF479673
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. M. Knapp, J. E. Ramsey, S.-X. Wang, A. R. Strauch, and R. J. Kelm Jr. Structure-Function Analysis of Mouse Pur II: CONFORMATION ALTERING MUTATIONS DISRUPT SINGLE-STRANDED DNA AND PROTEIN INTERACTIONS CRUCIAL TO SMOOTH MUSCLE {alpha}-ACTIN GENE REPRESSION J. Biol. Chem., December 7, 2007; 282(49): 35899 - 35909. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Knapp, J. E. Ramsey, S.-X. Wang, K. E. Godburn, A. R. Strauch, and R. J. Kelm Jr. Nucleoprotein Interactions Governing Cell Type-dependent Repression of the Mouse Smooth Muscle {alpha}-Actin Promoter by Single-stranded DNA-binding Proteins Pur{alpha} and Purbeta J. Biol. Chem., March 24, 2006; 281(12): 7907 - 7918. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Kelm Jr., S.-X. Wang, J. A. Polikandriotis, and A. R. Strauch Structure/Function Analysis of Mouse Pur{beta}, a Single-stranded DNA-binding Repressor of Vascular Smooth Muscle {alpha}-Actin Gene Transcription J. Biol. Chem., October 3, 2003; 278(40): 38749 - 38757. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Khalili, L. Del Valle, V. Muralidharan, W. J. Gault, N. Darbinian, J. Otte, E. Meier, E. M. Johnson, D. C. Daniel, Y. Kinoshita, et al. Pur{alpha} Is Essential for Postnatal Brain Development and Developmentally Coupled Cellular Proliferation As Revealed by Genetic Inactivation in the Mouse Mol. Cell. Biol., October 1, 2003; 23(19): 6857 - 6875. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Johnson, Y. Kinoshita, and D. C. Daniel A new member of the MCM protein family encoded by the human MCM8 gene, located contrapodal to GCD10 at chromosome band 20p12.3-13 Nucleic Acids Res., June 1, 2003; 31(11): 2915 - 2925. [Abstract] [Full Text] [PDF]
|
|