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Nucleic Acids Research, 2002, Vol. 30, No. 12 2609-2619
© 2002 Oxford University Press

Properties of triple helices formed by parallel-stranded hairpins containing 8-aminopurines

Anna Aviñó, Miriam Frieden, Juan Carlos Morales1, Beatriz García de la Torre1, Ramón Güimil García1, Ferran Azorín1, José Luis Gelpí2, Modesto Orozco2, Carlos González3 and Ramon Eritja1,*

Cygene Spain S.L., Parc Científic de Barcelona, Baldiri i Reixac 10-12, E-08028 Barcelona, Spain, 1Institut de Biologia Molecular de Barcelona, C.S.I.C., Jordi Girona 18-26, E-08034 Barcelona, Spain, 2Departament de Bioquímica i Biologia Molecular, Facultat de Química, Universitat de Barcelona, Martí i Franques 1, E-08028 Barcelona, Spain and 3Instituto de Estructura de la Materia, C.S.I.C., Serrano 119, E-28006 Madrid, Spain

Parallel-stranded hairpins with a polypyrimidine sequence linked to a complementary purine carrying 8-aminopurines such as 8-aminoadenine, 8-aminoguanine and 8-aminohypoxanthine bind polypyrimidine sequences complementary (in an antiparallel sense) to the purine part by a triple helix. The relative stabilities of triplexes were assessed by UV-absorption melting experiments as a function of pH and salt concentration. Hairpins carrying 8-aminopurines give very stable triple helical structures even at neutral pH, as confirmed by gel-shift experiments, circular dichroism and nuclear magnetic resonance spectroscopy. The modified hairpins may be redesigned to cope with small interruptions in the polypyrimidine target sequence.

* To whom correspondence should be addressed. Tel: +34 93 4006145; Fax: +34 93 2045904; Email: recgma@cid.csic.es


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