Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (693K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Wang, H.
Right arrow Articles by Lu, Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, H.
Right arrow Articles by Lu, Z.
Related Collections
Right arrow Polymorphism/mutation detection
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2002, Vol. 30, No. 12 e61
© 2002 Oxford University Press

Label-free hybridization detection of a single nucleotide mismatch by immobilization of molecular beacons on an agarose film

Hong Wang, Jiong Li, Heping Liu, Quanjun Liu, Qian Mei, Yijin Wang, Jijun Zhu, Nongyue He and Zuhong Lu*

Chien-Shiung Wu Laboratory, Department of Biomedical Engineering, Southeast University, Nanjing, China

We developed a new technique to immobilize a set of molecular beacons on an agarose film-coated slide and found that it has the ability to identify a single nucleotide difference in label-free DNA targets. The annealing properties, specificity and hybridization dynamics of the present technique were compared with those of the conventional technique that directly immobilizes molecular beacons on a planar glass slide. It is demonstrated that the molecular beacon array on an agarose film has high quench efficiency, an excellent discrimination ratio for single nucleotide mismatches and a short detection time. We hypothesize that such a low fluorescence background and high specificity molecular beacon array will find practical applications in label-free, high-throughput mutation analysis and disease diagnosis.

* To whom correspondence should be addressed. Tel: +86 25 3619983; Fax: +86 25 7712719; Email: zhlu{at}seu.edu.cn


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
E. A. Lukhtanov, S. G. Lokhov, V. V. Gorn, M. A. Podyminogin, and W. Mahoney
Novel DNA probes with low background and high hybridization-triggered fluorescence
Nucleic Acids Res., March 12, 2007; 35(5): e30 - e30.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
Q. Liu, Y. Bai, Q. Ge, S. Zhou, T. Wen, and Z. Lu
Microarray-in-a-Tube for Detection of Multiple Viruses
Clin. Chem., February 1, 2007; 53(2): 188 - 194.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
R. D. Stedtfeld, L. M. Wick, S. W. Baushke, D. M. Tourlousse, A. B. Herzog, Y. Xia, J. M. Rouillard, J. A. Klappenbach, J. R. Cole, E. Gulari, et al.
Influence of Dangling Ends and Surface-Proximal Tails of Targets on Probe-Target Duplex Formation in 16S rRNA Gene-Based Diagnostic Arrays
Appl. Envir. Microbiol., January 1, 2007; 73(2): 380 - 389.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Liu, H. Wang, Z. Shi, H. Wang, C. Yang, S. Silke, W. Tan, and Z. Lu
TaqMan probe array for quantitative detection of DNA targets
Nucleic Acids Res., January 10, 2006; 34(1): e4 - e4.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
B. Bockisch, T. Grunwald, E. Spillner, and R. Bredehorst
Immobilized stem-loop structured probes as conformational switches for enzymatic detection of microbial 16S rRNA
Nucleic Acids Res., June 24, 2005; 33(11): e101 - e101.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.