Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (349K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (36)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Liu, L.
Right arrow Articles by Kmiec, E. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, L.
Right arrow Articles by Kmiec, E. B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2002, Vol. 30, No. 13 2742-2750
© 2002 Oxford University Press

Rad51p and Rad54p, but not Rad52p, elevate gene repair in Saccharomyces cerevisiae directed by modified single-stranded oligonucleotide vectors

Li Liu, Shuqiu Cheng1, Anja J. van Brabant1 and Eric B. Kmiec*

Department of Biology, Delaware Biotechnology Institute, University of Delaware, 15 Innovation Way, Room 270, Newark, DE 19711, USA and 1 NaPro Genomics, 15 Innovation Way, Newark, DE 19711, USA

Synthetic single-stranded DNA vectors have been used to correct point and frameshift mutations in episomal or chromosomal targets in the yeast Saccharomyces cerevisiae. Certain parameters, such as the length of the vector and the genetic background of the organism, have a significant impact on the process of targeted gene repair, and point mutations are corrected at a higher frequency than frameshift mutations. Genetic analyses reveal that expression levels of the recombination/repair genes RAD51, RAD52 and RAD54 can affect the frequency of gene repair. Overexpression of RAD51 enhances the frequency 4-fold for correction of an episomal target and 5-fold for correction of a chromosomal target; overexpression of RAD54 is also effective in stimulating gene repair, to the same extent as RAD51 in the chromosomal target. In sharp contrast, RAD52 gene expression serves to reduce gene repair activity in rescue experiments and in experiments where RAD52 is overexpressed in a wild-type strain. This may suggest an antagonist role for Rad52p. Consistent with this notion, the highest level of targeted repair occurs when the RAD51 gene is overexpressed in a strain of yeast deficient in RAD52 gene function.

* To whom correspondence should be addressed. Tel: +1 302 831 3420; Fax: +1 302 831 3427; Email: ekmiec{at}udel.edu


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
X.-S. Wu, L. Xin, W.-X. Yin, X.-Y. Shang, L. Lu, R. M. Watt, K. S. E. Cheah, J.-D. Huang, D.-P. Liu, and C.-C. Liang
Increased efficiency of oligonucleotide-mediated gene repair through slowing replication fork progression
PNAS, February 15, 2005; 102(7): 2508 - 2513.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. Ferrara and E. B. Kmiec
Camptothecin enhances the frequency of oligonucleotide-directed gene repair in mammalian cells by inducing DNA damage and activating homologous recombination
Nucleic Acids Res., October 5, 2004; 32(17): 5239 - 5248.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
G. PAWELEC, E. MARIANI, J. McLEOD, A. BEN-YEHUDA, T. FULOP, M. ARINGER, and Y. BARNETT
Engineering Anticancer T Cells for Extended Functional Longevity
Ann. N.Y. Acad. Sci., June 1, 2004; 1019(1): 178 - 185.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. Liu, K. K. Maguire, and E. B. Kmiec
Genetic re-engineering of Saccharomyces cerevisiae RAD51 leads to a significant increase in the frequency of gene repair in vivo
Nucleic Acids Res., April 15, 2004; 32(7): 2093 - 2101.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Storici, C. L. Durham, D. A. Gordenin, and M. A. Resnick
Chromosomal site-specific double-strand breaks are efficiently targeted for repair by oligonucleotides in yeast
PNAS, December 9, 2003; 100(25): 14994 - 14999.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.