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Nucleic Acids Research, 2002, Vol. 30, No. 14 e64
© 2002 Oxford University Press

Rapid parallel mutation scanning of gene fragments using a microelectronic protein–DNA chip format

Heike A. Behrensdorf, Marc Pignot, Norbert Windhab and Andreas Kappel*

Nanogen Recognomics GmbH, Industriepark Hoechst, Building G830, 65926 Frankfurt am Main, Germany

*To whom correspondence should be addressed. Tel: +49 69 305 37285; Fax: +49 69 305 16009; Email: akappel{at}recognomics.com

We have developed a method for the de novo discovery of genetic variations, including single nucleotide polymorphisms and mutations, on microelectronic chip devices. The method combines the features of electronically controlled DNA hybridisation on open-format microarrays, with mutation detection by a fluorescence-labelled mismatch- binding protein. Electronic addressing of DNA strands to distinct test sites of the chip allows parallel analysis of several individuals, as demonstrated for mutations in different exons of the p53 gene. This microelectronic chip-based mutation discovery assay may substitute for time-consuming sequencing studies and will complement existing technologies in genomic research.


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