Nucleic Acids Research, 2002, Vol. 30, No. 15 3433-3442
© 2002 Oxford University Press
Evaluation of sequence motifs found in scaffold/matrix-attached regions (S/MARs)
1 Research Group Bioinformatics and 2 Research Group Epigenomics, Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-38124 Braunschweig, Germany and 3 BIOBASE GmbH, Halchtersche Straße 33, D-38304 Wolfenbüttel, Germany
*To whom correspondence should be addressed. Tel: +49 531 6181428; Fax: +49 531 6181266; Email: ili{at}gbf.de
Based on the contents of the database S/MARt DB, the most comprehensive data collection of scaffold/matrix-attached regions (S/MARs) publicly available thus far, we initiated a systematic evaluation of the stored data. By analyzing the 245 S/MAR sequences presently described in this database, we found that the S/MARs contained in this collection are generally AT-rich, with certain significant exceptions. Comparative analyses showed that most of the AT-rich motifs which were found to be enriched in S/MARs are also enriched in randomized S/MAR sequences of the same AT content. Some sequence patterns previously suggested to be characteristic for S/MARs were also investigated, among them potential binding sites for homeodomain transcription factors. Even though hexanucleotides containing the core motif of homeodomain factors were frequently observed in S/MARs, only a few potential binding sites for these factors were found enriched when compared with regulatory regions or exon sequences. All our analyses indicated that, on average, the observed frequency of motifs in S/MAR elements is largely influenced by the AT content. Our results can serve as a guideline for further improvements in the definition of S/MARs, which are now believed to constitute the functional coordinate system for genomic regulatory regions.
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