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Nucleic Acids Research, 2002, Vol. 30, No. 18 e95
© 2002 Oxford University Press

Rapid gene cloning using terminator primers and modular vectors

William F. Donahue, Brian M. Turczyk and Kevin A. Jarrell*

Department of Pharmacology and Experimental Therapeutics, Boston University Medical Center, 80 East Concord Street, Boston, MA 02118, USA

*To whom correspondence should be addressed at present address: Modular Genetics Inc., 65 Cummings Park, Woburn, MA 01801, USA. Tel: +1 781 271 9819; Fax: +1 781 271 9846; Email: kjarrell{at}modulargenetics.com

We seek to create useful biological diversity by exploiting the modular nature of genetic information. In this report we describe experiments that focus on the modular nature of plasmid cloning vectors. Bacterial plasmids are modular entities composed of origins of replication, selectable markers and other components. We describe a new ligation-independent cloning method that allows for rapid and seamless assembly of vectors from component modules. We further demonstrate that gene cloning can be accomplished simultaneously with assembly of a modular vector. This approach provides considerable flexibility as it allows for ‘menu driven’ cloning of genes into custom assembled modular vectors.


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