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Nucleic Acids Research, 2002, Vol. 30, No. 19 e97
© 2002 Oxford University Press

MagiProbe: a novel fluorescence quenching-based oligonucleotide probe carrying a fluorophore and an intercalator

Akio Yamane*

Institute for Medical Research, Wakunaga Pharmaceutical Co., Ltd, 1624 Shimokotachi, Kodacho, Takatagun, Hiroshima 739-1195, Japan

*Tel: +81 82645 2331; Fax: +81 82645 4351; Email: yamane_a{at}wakunaga.co.jp

Fluorescence is the favored signaling technology for molecular diagnoses. Fluorescence energy transfer-based methods are powerful homogeneous assay tools. A novel oligonucleotide probe, named MagiProbe, which is simple to use, is described, and information given about the duplex formed with a target. The probe internally has a fluorophore and an intercalator. Its fluorescence is quenched by the intercalator in the absence of a target sequence. On hybridization with a target sequence, the probe emits marked fluorescence due to the interference in quenching by intercalation. Furthermore, MagiProbe hybridized with a single-base mismatched target emits less fluorescence than with a perfect matched target. It therefore can detect a single base difference in a double-stranded form with a target.


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