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Nucleic Acids Research, 2002, Vol. 30, No. 2 e10
© 2002 Oxford University Press

DNA microarrays with PAMAM dendritic linker systems

Rüdiger Benters, Christof M. Niemeyer1,*, Denja Drutschmann1, Dietmar Blohm1 and Dieter Wöhrle2

Chimera Biotec GmbH, Schwachhauser Heerstrasse 30A, 28209 Bremen, Germany, 1Biotechnologie und Molekulare Genetik, University Bremen, FB 2 - UFT, PO Box 330440, 28334 Bremen, Germany and 2Institute of Organic and Macromolecular Chemistry, University Bremen, FB2, PO Box 330440, 28334 Bremen, Germany

The DNA microarray-based analysis of single nucleotide polymorphisms (SNPs) is important for the correlation of genetic variations and individual phenotypes, and for locating disease-causing genes. To facilitate the development of surfaces suitable for immobilization of oligonucleotides, we report here a novel method for the surface immobilization of DNA using pre-fabricated polyamidoamine (PAMAM) starburst dendrimers as mediator moieties. Dendrimers containing 64 primary amino groups in their outer sphere are covalently attached to silylated glass supports and, subsequently, the dendritic macromolecules are modified with glutaric anhydride and activated with N-hydroxysuccinimide. As a result of the dendritic PAMAM linker system the surfaces reveal both a very high immobilization efficiency for amino-modified DNA-oligomers, and also a remarkable high stability during repeated regeneration and re-using cycles. The performance of dendrimer-based DNA microarrays in the discrimination of SNPs is demonstrated.

* To whom correspondence should be addressed. Tel: +49 421 218 4911; Fax: +49 421 218 7578; Email: cmn{at}uni-bremen.de


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