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Nucleic Acids Research, 2002, Vol. 30, No. 20 4519-4526
© 2002 Oxford University Press

Complex formation of the neuron-specific ELAV-like Hu RNA-binding proteins

Katsumi Kasashima, Eiji Sakashita, Kuniaki Saito and Hiroshi Sakamoto*

Department of Biology, Graduate School of Science and Technology, Kobe University, 1-1 Rokkodaicho, Nadaku, Kobe 657-8501, Japan

*To whom correspondence should be addressed. Tel: +81 78 803 5796; Fax: +81 78 803 5720; Email: hsaka{at}kobe-u.ac.jp
Present address:
Eiji Sakashita, Department of Biochemistry, Jichi Medical School, Minamikawachi 329-0498, Japan

Hu proteins are RNA-binding proteins that are the vertebrate homologs of Drosophila ELAV, and are implicated in stabilization or enhanced translation of specific mRNAs with AU-rich elements (AREs) in the 3'-untranslated region. Here, using the yeast two-hybrid system, we show that neuron-specific Hu proteins can interact with themselves. Immuno precipitation assays demonstrated that the interaction between Hu proteins occurs in mammalian cells and is strongly enhanced in the presence of cellular RNA. Furthermore, using in situ chemical crosslinking assays, we found that HuD, one of the neuron-specific Hu proteins, multimerizes in cells. The crosslinked HuD multimers retain specific RNA-binding ability and can interact with additional Hu proteins. Consistent with this biochemical property, HuD showed granular distribution in two neurogenic cell lines. These results suggest that the RNA-bound form of HuD multimerizes cooperatively to form a specific granular structure that may serve as a site of post-transcriptional regulation of ARE-containing mRNAs.


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