Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (534K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Cox, J. C.
Right arrow Articles by Ellington, A. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cox, J. C.
Right arrow Articles by Ellington, A. D.
Related Collections
Right arrow Protein-nucleic acid interaction
Right arrow RNA characterisation and manipulation
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2002, Vol. 30, No. 20 e108
© 2002 Oxford University Press

Automated selection of aptamers against protein targets translated in vitro: from gene to aptamer

J. Colin Cox1, Andrew Hayhurst2, Jay Hesselberth3, Travis S. Bayer1, George Georgiou1,2 and Andrew D. Ellington*,1,3

1 Institute for Cellular and Molecular Biology, 2 Department of Chemical Engineering and 3 Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712, USA

*To whom correspondence should be addressed. Tel: +1 512 471 6445; Fax: +1 512 471 7014; Email: andy.ellington{at}mail.utexas.edu

Reagents for proteome research must of necessity be generated by high throughput methods. Apta mers are potentially useful as reagents to identify and quantitate individual proteins, yet are currently produced for the most part by manual selection procedures. We have developed automated selection methods, but must still individually purify protein targets. Therefore, we have attempted to select aptamers against protein targets generated by in vitro transcription and translation of individual genes. In order to specifically immobilize the protein targets for selection, they are also biotinylated in vitro. As a proof of this method, we have selected aptamers against translated human U1A, a component of the nuclear spliceosome. Selected sequences demonstrated exquisite mimicry of natural binding sequences and structures. These results not only reveal a potential path to the high throughput generation of aptamers, but also yield insights into the incredible specificity of the U1A protein for its natural RNA ligands.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 RNAHome page
G. Mayer, B. Wulffen, C. Huber, J. Brockmann, B. Flicke, L. Neumann, D. Hafenbradl, B. M. Klebl, M. J. Lohse, C. Krasel, et al.
An RNA molecule that specifically inhibits G-protein-coupled receptor kinase 2 in vitro
RNA, March 1, 2008; 14(3): 524 - 534.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. H. J. Bunka, B. J. Mantle, I. J. Morten, G. A. Tennent, S. E. Radford, and P. G. Stockley
Production and Characterization of RNA Aptamers Specific for Amyloid Fibril Epitopes
J. Biol. Chem., November 23, 2007; 282(47): 34500 - 34509.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
J. Konig, C. Julius, S. Baumann, M. Homann, H. U. Goringer, and M. Feldbrugge
Combining SELEX and the yeast three-hybrid system for in vivo selection and classification of RNA aptamers
RNA, April 1, 2007; 13(4): 614 - 622.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
F. Jarosch, K. Buchner, and S. Klussmann
In vitro selection using a dual RNA library that allows primerless selection
Nucleic Acids Res., July 19, 2006; 34(12): e86 - e86.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
T. S. BAYER, L. N. BOOTH, S. M. KNUDSEN, and A. D. ELLINGTON
Arginine-rich motifs present multiple interfaces for specific binding by RNA
RNA, December 1, 2005; 11(12): 1848 - 1857.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. Eulberg, K. Buchner, C. Maasch, and S. Klussmann
Development of an automated in vitro selection protocol to obtain RNA-based aptamers: identification of a biostable substance P antagonist
Nucleic Acids Res., March 3, 2005; 33(4): e45 - e45.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. A. Di Giusto and G. C. King
Construction, Stability, and Activity of Multivalent Circular Anticoagulant Aptamers
J. Biol. Chem., November 5, 2004; 279(45): 46483 - 46489.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Hu, J. Beck, and M. Nassal
SELEX-derived aptamers of the duck hepatitis B virus RNA encapsidation signal distinguish critical and non-critical residues for productive initiation of reverse transcription
Nucleic Acids Res., August 16, 2004; 32(14): 4377 - 4389.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Rajendran and A. D. Ellington
In vitro selection of molecular beacons
Nucleic Acids Res., October 1, 2003; 31(19): 5700 - 5713.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.