Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (161K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (16)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Bonnet-Corven, S.
Right arrow Articles by Osborne, H. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bonnet-Corven, S.
Right arrow Articles by Osborne, H. B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 2002, Vol. 30, No. 21 4667-4674
© 2002 Oxford University Press

An analysis of the sequence requirements of EDEN-BP for specific RNA binding

Sylvie Bonnet-Corven, Yann Audic, Francis Omilli and H. Beverley Osborne*

UMR 6061 CNRS–Génétique et Développement, IFR 97, Université de Rennes 1, Faculté de Médecine, 2 Avenue Léon Bernard, CS 34317, 35043 Rennes Cedex, France

*To whom correspondence should be addressed. Tel: +33 223 23 45 23; Fax: +33 223 23 44 78; Email: beverley.osborne{at}univ-rennes1.fr

EDEN-BP (embryo deadenylation element-binding protein) binds specifically to the EDEN motif in the 3'-untranslated regions of maternal mRNAs and targets these mRNAs for deadenylation and translational repression in Xenopus laevis embryos. EDEN-BP contains three RNA recognition motifs (RRMs) and is related to the elav family of RNA-binding proteins. In the present study we show that the two N-terminal RRMs of EDEN-BP are necessary for the interaction with EDEN as well as a part of the linker region (between RRM2 and RRM3). Using a band shift assay we show that two different complexes are formed according to the size and, therefore, the functional nature of the EDEN motif. Finally, we show that EDEN-BP can form a dimer in a two-hybrid assay. Accordingly, we suggest that the functional configuration of EDEN-BP is a dimer.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J BiochemHome page
D. Mori, N. Sasagawa, Y. Kino, and S. Ishiura
Quantitative Analysis of CUG-BP1 Binding to RNA Repeats
J. Biochem., March 1, 2008; 143(3): 377 - 383.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
K. C.M. Moraes, C. J. Wilusz, and J. Wilusz
CUG-BP binds to RNA substrates and recruits PARN deadenylase
RNA, June 1, 2006; 12(6): 1084 - 1091.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Han and T. A. Cooper
Identification of CELF splicing activation and repression domains in vivo
Nucleic Acids Res., May 13, 2005; 33(9): 2769 - 2780.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. A. Faustino and T. A. Cooper
Identification of Putative New Splicing Targets for ETR-3 Using Sequences Identified by Systematic Evolution of Ligands by Exponential Enrichment
Mol. Cell. Biol., February 1, 2005; 25(3): 879 - 887.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Delaunay, G. L. Mee, N. Ezzeddine, G. Labesse, C. Terzian, M. Capri, and O. Ait-Ahmed
The Drosophila Bruno paralogue Bru-3 specifically binds the EDEN translational repression element
Nucleic Acids Res., June 4, 2004; 32(10): 3070 - 3082.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
G. Singh, N. Charlet-B., J. Han, and T. A. Cooper
ETR-3 and CELF4 protein domains required for RNA binding and splicing activity in vivo
Nucleic Acids Res., February 18, 2004; 32(3): 1232 - 1241.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.