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Nucleic Acids Research, 2002, Vol. 30, No. 21 4709-4719
© 2002 Oxford University Press

Translational fusions with the engrailed repressor domain efficiently convert plant transcription factors into dominant-negative functions

Heike Markel, John Chandler and Wolfgang Werr*

Institut für Entwicklungsbiologie Universität zu Köln, 50923 Köln, Germany

*To whom correspondence should be addressed. Tel: +49 221 470 2619; Fax: +49 221 470 5164; Email: werr{at}uni-koeln.de

Evidence is provided that plant transcription factors can be efficiently reprogrammed to dominant- negative functions by the use of a repressor domain of the engrailed (en) gene from Drosophila. Ectopic expression of translational fusions between the en298 N-terminus and the complete coding regions of the SHOOTMERISTEMLESS, APETALA3, PISTILLATA and KNAT1 transcription factors results in trans-dominant functions which phenocopy loss-of-function mutants. The combination of the dominant-negative en298-STM function with the hormone-binding domain of the glucocorticoid receptor provides strong evidence that phenocopies rely on the incorporation of the chimeric protein into the nuclear compartment. By this dominant-negative approach KNAT1 was rapidly identified to be encoded by the BREVIPEDICELLUS locus. Dominant-negative chimeric proteins may be of wide use to elucidate biological functions of plant transcriptional activators and may be suitable to study protein–protein interactions in planta.


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